Bu H Y, Jing J Z, Jia J F
Department of Biology, Northwest University, Xi'an 710069.
Shi Yan Sheng Wu Xue Bao. 2001 Jun;34(2):81-7.
The regenerated shoot segments of Alhagi pseudalhagi were sliced and infected with Agrobacterium rhizogenes strain A4. The hairy roots and transformed calli were obtained through selection on hormone free MS medium. The transformants were cultured on MS medium with 2 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5-1 mg/L 6-benzylaminopurine (6-BA) to induce calli. 3 mg/L 6-BA and 0.5 mg/L naphthalene acetic acid (NAA) were applied for shoot differentiation. Shoots were planted on MS medium with 2 mg/L indole-3-butyric acid (IBA) and produced roots. Opine analysis proved the integration and expression of T-DNA in over 95% hairy roots, 75% transformed calli and transformed plantlets respectively. The 81% hairy root cells had normal chromosome numbers (2n = 18). The alterations of chromosome number were observed. After one year of subculturing, the regeneration ability of transformants was maintained.
将骆驼刺再生的茎段切片，用发根农杆菌A4菌株侵染。通过在无激素的MS培养基上筛选获得毛状根和转化愈伤组织。将转化体在含有2 mg/L 2,4-二氯苯氧乙酸（2,4-D）和0.5-1 mg/L 6-苄基腺嘌呤（6-BA）的MS培养基上培养以诱导愈伤组织。使用3 mg/L 6-BA和0.5 mg/L萘乙酸（NAA）进行芽分化。将芽接种在含有2 mg/L吲哚-3-丁酸（IBA）的MS培养基上诱导生根。冠瘿碱分析证明T-DNA分别在超过95%的毛状根、75%的转化愈伤组织和转化植株中整合并表达。81%的毛状根细胞染色体数目正常（2n = 18）。观察到染色体数目的变化。继代培养一年后，转化体的再生能力得以维持。
