He Y K
Department of Horticulture, Northwestern Agricultural University, Yangling, Shanxi.
Chin J Biotechnol. 1990;6(2):131-7.
Ri T-DNA transformed roots were induced out on hypocotyl segments of cabbage infected with Agrobacterium rhizogenes. After cultured for four months on solid MS medium without hormones, some parts of a few of transformed roots were dedifferentiated into callus clumps, and then redifferentiated into shoot buds. On solid MS medium supplemented with kinetin, shoot buds were differentiated directly from transformed roots without callus formation. Among several concentrations of kinetin, 14 microM gave rise to the best results for differentiation of shoot buds. In liquid MS medium, 4.5 microM of 6-benzylaminopurine (BA) also showed the increase in the number of shoot buds from transformed roots. All of these shoot buds were able to grow further, root out on root induction medium, and develop into young plants.
发根农杆菌感染的甘蓝下胚轴切段上诱导出了Ri T-DNA转化根。在不含激素的固体MS培养基上培养四个月后，少数转化根的部分脱分化形成愈伤组织块，然后再分化形成芽。在添加激动素的固体MS培养基上，转化根直接分化形成芽，不形成愈伤组织。在几种浓度的激动素中，14微摩尔/升对芽的分化效果最佳。在液体MS培养基中，4.5微摩尔/升的6-苄基腺嘌呤（BA）也能使转化根产生更多的芽。所有这些芽都能进一步生长，在生根诱导培养基上生根，并发育成幼苗。
