[Research on the regulation of glucoamylase gene(glaA) expression in A. niger. II. Analysis of the function of 5'-regulatory region of A. niger T21 and 3.795 glaA gene].

Two plasmid vectors pXH2 and pGH1 were constructed through the fusion of E. coli hph gene, the report gene and the 5' upstream regions of A. niger T21 and 3.795 respectively, as well as the terminator of A. nidulans trpC gene. The plasmid vectors were than used to transform A. niger T21 to functionally identify those different basic groups between the two 5' upstream regions responsible for high-level expression of the glaA gene. Southern analysis of two transformants XH2C and GH1C revealed that pXH2 and pGH1 were integrated respectively into the chromosome at same site with two copies in tandem array. The level resistant to HmB(3000 micrograms/ml) of XH2C was twice as high as that (1500 micrograms/ml) of GH1C, indicating that the changes of basic groups through mutation result in twice increase of functional level of region responsible for transcription and regulation of A. niger T21 glaA gene compared with that of 3.795.