Tang G, Yang K
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Chin J Biotechnol. 1995;11(4):237-41.
Starch digestible delta-integrants were constructed by integrative transformation of a linear YIP plasmid carrying Aspergillus niger glucoamylase cDNA under the control of the MF alpha 1 promoter and its prepro signal and the delta sequence of the Ty element from yeast. The integration of glucoamylase cDNA into Saccharomyces cerevisiae was identified by Southern analysis. The secreted glucoamylase activity of integrants in the medium with soluble starch as a carbon source reached 2.5 u/ml. After ten times of successive transfers in a nonselective medium, the activity of secreted glucoamylase of integrant was approximately at its original level.
通过将携带黑曲霉葡糖淀粉酶cDNA（在MFα1启动子及其前原信号以及来自酵母的Ty元件的δ序列控制下）的线性YIP质粒进行整合转化，构建了可消化淀粉的δ整合体。通过Southern分析鉴定葡糖淀粉酶cDNA整合到酿酒酵母中。以可溶性淀粉作为碳源的培养基中，整合体分泌的葡糖淀粉酶活性达到2.5 U/ml。在非选择性培养基中连续传代十次后，整合体分泌的葡糖淀粉酶活性大致维持在其原始水平。
