Bagwe B A, Sharma S M
J Nucl Med. 1976 Apr;17(4):313-6.
Leucocytes were separated from rats' blood by dextran sedimentation method and labeled with 99mTc-pertechnetate. A maximum labeling efficiency of 18.5% was achieved with the addition of 1 mg of stannous chloride. The labeled leucocytes were intact and viable and the 99mTc label was firm. Distribution studies in rats, 3 hr after injection of 99mTc-labeled leucocytes, showed 33% concentration in the mineral part of the bone, this being slightly lower than the 36.8% concentration in the liver. Technetium concentration in the bones due to a complex formed by various ingredients used for leucocyte labeling was excluded since negligible bone uptake was noted after injection of an incubated mixture of 99mTc and all ingredients except leucocytes. Also 51Cr-labeled leucocytes showed no localization in the bone. These studies indicate that 99mTc and intact leucocytes form some sort of complex which has an affinity for bone cortex.
采用葡聚糖沉降法从大鼠血液中分离白细胞，并用高锝[99mTc]酸盐进行标记。加入1mg氯化亚锡后，标记效率最高可达18.5%。标记的白细胞完整且有活力，99mTc标记牢固。给大鼠注射99mTc标记的白细胞3小时后进行分布研究，结果显示骨矿物质部分的浓度为33%，略低于肝脏中的浓度（36.8%）。由于注射99mTc与除白细胞外的所有成分的孵育混合物后骨摄取可忽略不计，因此排除了因白细胞标记所用各种成分形成的复合物导致的骨中锝浓度升高的情况。此外，51Cr标记的白细胞在骨中无定位。这些研究表明，99mTc与完整的白细胞形成了某种对骨皮质有亲和力的复合物。
