Wasserman R H, Corradino R A, Krook L, Hughes M R, Haussler M R
J Nutr. 1976 Apr;106(4):457-65. doi: 10.1093/jn/106.4.457.
Cestrum diurnum (day-blooming jessamine) has been proposed to cause calcinosis in horses and cattle in Florida. The present studies investigated some physiological properties of the plant, using the chick as the experimental animal. The inclusion of dried leaf powder in a rachitogenic diet restored intestinal calcium-binding protein synthesis (CaBP) and increased calcium absorption in the cholecalciferol-deficient chick. The estimated level of cholecalciferol-equivalents in the dried leaf was about 30,000 to 35,000 IU/kg. Most of the activity was extractable with methanol:chloroform (2:1), indicating that the major cholecalciferol-like component in C. diurnum was different from the water soluble factor(s) in Solanum malacoxylon. The time course of effect of C. diurnum extract in rachitic chicks was similar to that ot 1,25-dihydroxycholecalciferol but the former had a longer lag time. The strontium fed chick, in which the kidney 25-hydroxycholecalciferol-1alpha-hydroxylase is inhibited, responded to C. diurnum extract, confirming the 1alpha,25-dihydroxycholecalciferol-like character of the Cestrum factor. The extract also appeared to interact with the intestinal 1 alpha,25-dihydroxycholecalciferol cytosol receptor although this observation is preliminary. These findings indicate that the l alpha,25-dihydroxycholecalciferol-like principle in C. diurnum many cause excessive calcium and phosphate absorption leading to calcinosis.
白天开花的夜香树被认为会导致佛罗里达州的马和牛患钙质沉着症。本研究以雏鸡作为实验动物，对该植物的一些生理特性进行了研究。在致佝偻病饮食中添加干叶粉可恢复肠道钙结合蛋白的合成（CaBP），并增加维生素D缺乏雏鸡的钙吸收。干叶中维生素D当量的估计水平约为30,000至35,000 IU/kg。大部分活性可用甲醇：氯仿（2:1）提取，这表明夜香树中主要的维生素D样成分与茄属植物软刺茄中的水溶性因子不同。夜香树提取物对佝偻病雏鸡的作用时间进程与1,25-二羟基维生素D相似，但前者的滞后时间更长。饲喂锶的雏鸡，其肾脏25-羟基维生素D-1α-羟化酶受到抑制，但对夜香树提取物有反应，这证实了夜香树因子具有1α,25-二羟基维生素D样特性。该提取物似乎还与肠道1α,25-二羟基维生素D胞质受体相互作用，不过这一观察结果尚属初步。这些发现表明，夜香树中类似1α,25-二羟基维生素D的成分可能会导致钙和磷过度吸收，从而引发钙质沉着症。
