[反相高效液相色谱法拆分拉贝洛尔对映体]
[Separation of enantiomeric labetalol by reversed-phase high performance liquid chromatography].
作者信息
Zhao H, Li H, Qui Z
机构信息
Chongqing University of Medical Sciences, Chongqing, 400046.
出版信息
Se Pu. 1999 Jul;17(4):369-71.
A reversed-phase high performance liquid chromatographic method for the separation of labetalol enantiomers was developed. In the method, 2, 3, 4, 6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate (GITC) was chosen as the reagent for pre-column chiral derivatization of labetalol to give four diastereomeric thiourea derivatives. These derivatives were efficiently separated on a Nova-Pak C18 column using V(MeOH):V(0.01 mol.L-1(pH 7.00) H2PO4(-)-HPO(4)2- buffer) = 51:49 as the mobile phase and detected by UV detector at a wavelength of 250 nm or fluorescence detector at lambda ex = 340 nm and lambda em = 440 nm. The effects of the pH of mobile phase on the retention and fluorescence absorbance are also discussed.
建立了一种反相高效液相色谱法用于分离拉贝洛尔对映体。该方法选用2,3,4,6-四-O-乙酰基-β-D-吡喃葡萄糖基异硫氰酸酯（GITC）作为柱前手性衍生化试剂，使拉贝洛尔生成四种非对映体硫脲衍生物。这些衍生物在Nova-Pak C18柱上以V（甲醇）:V（0.01 mol·L⁻¹（pH 7.00）磷酸二氢钠-磷酸氢二钠缓冲液）= 51:49作为流动相进行有效分离，并通过紫外检测器在250 nm波长处检测，或通过荧光检测器在激发波长λex = 340 nm和发射波长λem = 440 nm处检测。还讨论了流动相pH值对保留时间和荧光吸收的影响。
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