Berthault F, Kintz P, Mangin P
Institut de Médecine Légale, Strasbourg.
Ann Pharm Fr. 1997;55(1):12-9.
Determination of optical isomers is based on some molecule properties to have one or more chiral asymmetrical carbon center. Enantiomers can be resolved by high performance liquid chromatography (HPLC) with a chiral mobile phase additive, by change into diastereoisomers by derivatization or by separation on chiral column. We retained the last technique for the separation of seven beta-blockers (atenolol, alprenolol, labetalol, nadolol, oxprenolol, pindolol and propranolol) using an alpha 1-AGP column. Results indicate that the mobile phase was specific to each beta-blocker. The wavelength chosen was lambda = 225 nm and the flow rate was 0.9 ml/min. A part from that, determinations of isomers of propranolol and atenolol in different fluids biologicals were also achieved.
光学异构体的测定基于某些分子具有一个或多个手性不对称碳中心的性质。对映体可以通过使用手性流动相添加剂的高效液相色谱（HPLC）、通过衍生化转化为非对映异构体或在手性柱上进行分离来拆分。我们采用最后一种技术，使用α1-AGP柱分离七种β受体阻滞剂（阿替洛尔、阿普洛尔、拉贝洛尔、纳多洛尔、氧烯洛尔、吲哚洛尔和普萘洛尔）。结果表明，流动相对每种β受体阻滞剂具有特异性。选择的波长为λ = 225 nm，流速为0.9 ml/min。除此之外，还实现了不同生物流体中普萘洛尔和阿替洛尔异构体的测定。
