Wang Ching Y, Jones Richard F, Debiec-Rychter Maria, Soos Gyorgyike, Haas Gabriel P
Department of Urology, SUNY Upstate Medical University, VA Medical Center, Syracuse, NY, USA.
Anticancer Res. 2002 Nov-Dec;22(6B):3529-35.
The arylamine N-acetyltransferases play a major role in the metabolic activation of carcinogenic amines that are present in cigarette smoke and a variety of other exogenous sources. The objective of this study was to determine the association of rapid or slow arylamine N-acetyltransferase (NAT) genotypes with smoking history and the risk for developing both bladder and prostate cancer.
The subjects analyzed were a case group of 17 double-cancer patients and 34 age-matched controls who had benign prostatic hypertrophy, but were asymptomatic for prostate or bladder cancers. Genotyping of NAT1 and NAT2 alleles was done by restriction fragment length polymorphism and/or sequencing of NAT genes amplified from genomic DNAs by the polymerase chain reaction (PCR).
No significant correlation was found between NAT1 genotypes, double cancer, and smoking history. While NAT2-smoking interaction gave an odds ratio of only 1.70 (p = 0.117), a disproportionate number of cancer cases were genotypically rapid: 12 of 17 cancer cases vs. 13 of 34 controls (odds ratio 3.88; p = 0.040).
Rapid NAT2 genotype correlated significantly with the development of double prostate-bladder cancer.
芳胺N - 乙酰基转移酶在香烟烟雾及多种其他外源性来源中存在的致癌胺的代谢活化过程中起主要作用。本研究的目的是确定芳胺N - 乙酰基转移酶（NAT）快速或慢速基因型与吸烟史以及发生膀胱癌和前列腺癌风险之间的关联。
所分析的受试者包括一个病例组，由17例双癌患者组成，以及34例年龄匹配的对照，这些对照患有良性前列腺增生，但无前列腺癌或膀胱癌症状。通过聚合酶链反应（PCR）从基因组DNA扩增的NAT基因进行限制性片段长度多态性分析和/或测序，对NAT1和NAT2等位基因进行基因分型。
未发现NAT1基因型、双癌与吸烟史之间存在显著相关性。虽然NAT2与吸烟的相互作用的优势比仅为1.70（p = 0.117），但从基因型来看，快速型在癌症病例中的比例过高：17例癌症病例中有12例，而34例对照中有13例（优势比3.88；p = 0.040）。
NAT2快速基因型与前列腺 - 膀胱癌的发生显著相关。
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