Schubert Patricia, Schantz Michele M, Sander Lane C, Wise Stephen A
Analytical Chemistry Division, National Institute of Standards and Technology, 100 Bureau Drive Stop 8392, Gaithersburg, Maryland 20899-8392, USA.
Anal Chem. 2003 Jan 15;75(2):234-46. doi: 10.1021/ac0259111.
An analytical approach based on gas chromatography/ mass spectrometry (GC/MS) is presented for the measurement of polycyclic aromatic hydrocarbons with molecular weight (MW) 300 and 302 in environmental samples. Three different GC stationary phases [5% and 50% phenyl methylpolysiloxane and dimethyl (50% liquid crystalline) polysiloxane] were compared, and retention indexes (RI) are given for 23 individual MW 302 isomers. Identification of MW 300 and 302 isomers in four environmental-matrix Standard Reference Materials (SRMs) (SRM 1597, coal tar extract; SRM 1648 and SRM 1649a, air particulate matter; and SRM 1941, marine sediment) was based on the comparison of RI data and mass spectra from authentic standards. Dibenzo[a,l]pyrene, which is of considerable interest because of its high carcinogenicity, was identified and quantified in the four environmental-matrix SRMs. A total of 23 isomers of MW 302 and four isomers of MW 300 were quantified in four different environmental-matrix SRMs, and the results are compared to previously reported results based on liquid chromatography with fluorescence detection.
本文介绍了一种基于气相色谱/质谱联用（GC/MS）的分析方法，用于测定环境样品中分子量为300和302的多环芳烃。比较了三种不同的气相色谱固定相[5%和50%苯基甲基聚硅氧烷以及二甲基（50%液晶）聚硅氧烷]，并给出了23种分子量为302的单个异构体的保留指数（RI）。通过比较RI数据和来自标准品的质谱图，对四种环境基质标准参考物质（SRM）[SRM 1597，煤焦油提取物；SRM 1648和SRM 1649a，空气颗粒物；以及SRM 1941，海洋沉积物]中分子量为300和302的异构体进行了鉴定。由于其高致癌性而备受关注的二苯并[a,l]芘，在这四种环境基质SRM中被鉴定并定量。在四种不同的环境基质SRM中对总共23种分子量为302的异构体和四种分子量为300的异构体进行了定量，并将结果与先前基于液相色谱荧光检测报道的结果进行了比较。
