古细菌中无开放阅读框且编码逆转录酶的II组内含子，具有归巢至相关II组内含子开放阅读框的模式。
ORF-less and reverse-transcriptase-encoding group II introns in archaebacteria, with a pattern of homing into related group II intron ORFs.
作者信息
Dai Lixin, Zimmerly Steven
出版信息
RNA. 2003 Jan;9(1):14-9. doi: 10.1261/rna.2126203.
Abstract
Although group II intron retroelements are prevalent in eubacteria, they have not been identified in archaebacteria in the first 10 genomes sequenced. However, the recently sequenced archael genome of Methanosarcina acetivorans contains 21 group II introns, including 7 introns that do not encode reverse transcriptase ORFs. To our knowledge, these are the first retroelements identified in archaebacteria, and the first ORF-less group II introns in bacteria. Furthermore, the insertion pattern of the introns is highly unusual. The introns appear to insert site-specifically into ORFs of other group II introns, forming nested clusters of up to four introns, but there are no flanking exons that could encode a functional protein after the introns have been spliced out.
摘要
虽然II组内含子反转录元件在真细菌中普遍存在，但在前10个已测序的古细菌基因组中尚未发现它们。然而，最近测序的嗜乙酰甲烷八叠球菌古细菌基因组包含21个II组内含子，其中7个内含子不编码逆转录酶开放阅读框。据我们所知，这些是在古细菌中鉴定出的首批反转录元件，也是细菌中首批无开放阅读框的II组内含子。此外，内含子的插入模式非常不寻常。这些内含子似乎以位点特异性方式插入到其他II组内含子的开放阅读框中，形成多达四个内含子的嵌套簇，但在这些内含子被剪接出去后，没有侧翼外显子能够编码功能性蛋白质。
相似文献
1
ORF-less and reverse-transcriptase-encoding group II introns in archaebacteria, with a pattern of homing into related group II intron ORFs.古细菌中无开放阅读框且编码逆转录酶的II组内含子，具有归巢至相关II组内含子开放阅读框的模式。
RNA. 2003 Jan;9(1):14-9. doi: 10.1261/rna.2126203.
2
Coevolution of group II intron RNA structures with their intron-encoded reverse transcriptases.II类内含子RNA结构与其内含子编码的逆转录酶的共同进化。
RNA. 2001 Aug;7(8):1142-52. doi: 10.1017/s1355838201010251.
3
Multiple homing pathways used by yeast mitochondrial group II introns.酵母线粒体II类内含子所使用的多种归巢途径。
Mol Cell Biol. 2000 Nov;20(22):8432-46. doi: 10.1128/MCB.20.22.8432-8446.2000.
4
Evolution of rbcL group IA introns and intron open reading frames within the colonial Volvocales (Chlorophyceae).团藻目（绿藻纲）中rbcL基因IA组内含子及内含子开放阅读框的进化
Mol Phylogenet Evol. 2002 Jun;23(3):326-38. doi: 10.1016/s1055-7903(02)00030-1.
5
Evolutionary origin of a plant mitochondrial group II intron from a reverse transcriptase/maturase-encoding ancestor.来自逆转录酶/成熟酶编码祖先的植物线粒体II类内含子的进化起源
J Plant Res. 2006 Jul;119(4):363-71. doi: 10.1007/s10265-006-0284-0. Epub 2006 Jun 9.
6
Compilation and analysis of group II intron insertions in bacterial genomes: evidence for retroelement behavior.细菌基因组中II组内含子插入的汇编与分析：反转录元件行为的证据
Nucleic Acids Res. 2002 Mar 1;30(5):1091-102. doi: 10.1093/nar/30.5.1091.
7
A broadscale phylogenetic analysis of group II intron RNAs and intron-encoded reverse transcriptases.对 II 组内含子 RNA 和内含子编码的逆转录酶进行广泛的系统发育分析。
Mol Biol Evol. 2009 Dec;26(12):2795-808. doi: 10.1093/molbev/msp193. Epub 2009 Aug 27.
8
Group II introns in eubacteria and archaea: ORF-less introns and new varieties.真细菌和古细菌中的II类内含子：无开放阅读框的内含子及新变体。
RNA. 2008 Sep;14(9):1704-13. doi: 10.1261/rna.1056108. Epub 2008 Aug 1.
9
A group II intron-type open reading frame from the thermophile Bacillus (Geobacillus) stearothermophilus encodes a heat-stable reverse transcriptase.嗜热脂肪芽孢杆菌（嗜热栖热菌属）的一个II组内含子型开放阅读框编码一种热稳定逆转录酶。
Appl Environ Microbiol. 2004 Dec;70(12):7140-7. doi: 10.1128/AEM.70.12.7140-7147.2004.
10
The highly variable mitochondrial small-subunit ribosomal RNA gene of Ophiostoma minus.微小亚单位线粒体核糖体 RNA 基因的高度变异性。
Fungal Biol. 2011 Nov;115(11):1122-37. doi: 10.1016/j.funbio.2011.07.007. Epub 2011 Jul 23.
引用本文的文献
1
Protein-free catalysis of DNA hydrolysis and self-integration by a ribozyme.一种核酶对DNA水解和自我整合的无蛋白质催化作用。
Nucleic Acids Res. 2025 Jan 11;53(2). doi: 10.1093/nar/gkae1224.
2
Engineered CRISPR prime editors with compact, untethered reverse transcriptases.工程化的 CRISPR 引物编辑器，具有紧凑、无束缚的逆转录酶。
Nat Biotechnol. 2023 Mar;41(3):337-343. doi: 10.1038/s41587-022-01473-1. Epub 2022 Sep 26.
3
Distinct Expansion of Group II Introns During Evolution of Prokaryotes and Possible Factors Involved in Its Regulation.原核生物进化过程中II组内含子的独特扩增及其调控的可能相关因素
Front Microbiol. 2022 Feb 28;13:849080. doi: 10.3389/fmicb.2022.849080. eCollection 2022.
4
Mitogenome of a stink worm (Annelida: Travisiidae) includes degenerate group II intron that is also found in five congeneric species.一个臭虫（环节动物：Travisiidae）的线粒体基因组包含退化的组 II 内含子，该内含子也存在于五个同种物种中。
Sci Rep. 2022 Mar 15;12(1):4449. doi: 10.1038/s41598-022-08103-5.
5
Retrotransposons as pathogenicity factors of the plant pathogenic fungus Botrytis cinerea.逆转座子作为植物病原菌 Botrytis cinerea 的致病性因子。
Genome Biol. 2021 Aug 16;22(1):225. doi: 10.1186/s13059-021-02446-4.
6
Prokaryotic reverse transcriptases: from retroelements to specialized defense systems.原核生物逆转录酶：从逆转座子到专门的防御系统。
FEMS Microbiol Rev. 2021 Nov 23;45(6). doi: 10.1093/femsre/fuab025.
7
Systematic prediction of genes functionally associated with bacterial retrons and classification of the encoded tripartite systems.系统预测与细菌反转录酶功能相关的基因，并对编码的三分体系统进行分类。
Nucleic Acids Res. 2020 Dec 16;48(22):12632-12647. doi: 10.1093/nar/gkaa1149.
8
Division of labour in a matrix, rather than phagocytosis or endosymbiosis, as a route for the origin of eukaryotic cells.细胞内共生或吞噬作用，而是通过细胞的“分工合作”，作为真核细胞起源的一种途径。
Biol Direct. 2020 Apr 28;15(1):8. doi: 10.1186/s13062-020-00260-9.
9
Contribution of Mobile Group II Introns to Genome Evolution.移动II类内含子对基因组进化的贡献。
Front Microbiol. 2018 Apr 4;9:627. doi: 10.3389/fmicb.2018.00627. eCollection 2018.
10
Chloroplast genome expansion by intron multiplication in the basal psychrophilic euglenoid .基部嗜冷裸藻中内含子倍增导致叶绿体基因组扩张
PeerJ. 2017 Aug 25;5:e3725. doi: 10.7717/peerj.3725. eCollection 2017.
本文引用的文献
1
Identification of a family of group II introns encoding LAGLIDADG ORFs typical of group I introns.鉴定出一个编码I类内含子典型的LAGLIDADG开放阅读框的II类内含子家族。
RNA. 2002 Nov;8(11):1373-7. doi: 10.1017/s1355838202023087.
2
Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1.嗜热蓝藻伸长聚球藻BP-1的全基因组结构
DNA Res. 2002 Aug 31;9(4):123-30. doi: 10.1093/dnares/9.4.123.
3
The genome of M. acetivorans reveals extensive metabolic and physiological diversity.嗜乙酸甲烷杆菌的基因组揭示了广泛的代谢和生理多样性。
Genome Res. 2002 Apr;12(4):532-42. doi: 10.1101/gr.223902.
4
Compilation and analysis of group II intron insertions in bacterial genomes: evidence for retroelement behavior.细菌基因组中II组内含子插入的汇编与分析：反转录元件行为的证据
Nucleic Acids Res. 2002 Mar 1;30(5):1091-102. doi: 10.1093/nar/30.5.1091.
5
RNAMotif, an RNA secondary structure definition and search algorithm.RNA基序，一种RNA二级结构定义及搜索算法。
Nucleic Acids Res. 2001 Nov 15;29(22):4724-35. doi: 10.1093/nar/29.22.4724.
6
Coevolution of group II intron RNA structures with their intron-encoded reverse transcriptases.II类内含子RNA结构与其内含子编码的逆转录酶的共同进化。
RNA. 2001 Aug;7(8):1142-52. doi: 10.1017/s1355838201010251.
7
The ins and outs of group II introns.II类内含子的来龙去脉。
Trends Genet. 2001 Jun;17(6):322-31. doi: 10.1016/s0168-9525(01)02324-1.
8
Phylogenetic relationships among group II intron ORFs.第二组内含子开放阅读框之间的系统发育关系。
Nucleic Acids Res. 2001 Mar 1;29(5):1238-50. doi: 10.1093/nar/29.5.1238.
9
Bioenergetic aspects of halophilism.嗜盐性的生物能量学方面
Microbiol Mol Biol Rev. 1999 Jun;63(2):334-48. doi: 10.1128/MMBR.63.2.334-348.1999.
10
Group II and group III introns of twintrons: potential relationships with nuclear pre-mRNA introns.双内含子的II组和III组内含子：与核前体mRNA内含子的潜在关系。
Trends Biochem Sci. 1993 Dec;18(12):467-71. doi: 10.1016/0968-0004(93)90008-b.
Zotero 插件