[从鸡眼草属和木蓝属植物分离的根瘤菌的分类学研究]

[A study on taxonomy of rhizobia isolated from Kummerwia sp. and Indigofera sp].

作者信息

Wei G, Chen W, Zhu M

机构信息

Dept Resource and Environment, Northwest Agricultural University, Yangling 712100.

出版信息

Wei Sheng Wu Xue Bao. 1999 Oct;39(5):387-95.

PMID:12555518

Abstract

The rhizobial strains isolated from Kummerowia and Indigofera and the known reference strains were classified by performing numerical taxonomy. New isolated strains were divided into two new clusters at 83% similarity level. Based on the numerical taxonomy, additional isolates in each cluster were studied by using SDS-PAGE of whole-cell protein. Twenty-four strains isolated from Kummerowia fell into cluster 1. Twenty strains isolated from Indigofera fell into cluster 2. The results of G + C mol% and DNA homology analysis showed that the DNA homologies between the central strain SH713 and SHL042 and the 13 type strains were less than 61%. Thus, the rhizobial strains from Kummerowia and Indigofera were two new individual species of Rhizobium.

摘要

对从鸡眼草属（Kummerowia）和木蓝属（Indigofera）分离得到的根瘤菌菌株以及已知参考菌株进行数值分类。新分离的菌株在83%的相似性水平上被分为两个新的类群。基于数值分类，通过全细胞蛋白的SDS-PAGE对每个类群中的其他分离株进行了研究。从鸡眼草属分离的24株菌株归入类群1。从木蓝属分离的20株菌株归入类群2。G + C mol%和DNA同源性分析结果表明，中心菌株SH713和SHL042与13株模式菌株之间的DNA同源性低于61%。因此，来自鸡眼草属和木蓝属的根瘤菌菌株是根瘤菌属的两个新的独立物种。