[嗜酸丛毛单胞菌AN3降解苯胺代谢途径的研究]

[Studies on the metabolism pathway of aniline degradation by Comamonas acidovorans AN3].

作者信息

Liu Z, Yang H, Zhou P

机构信息

Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.

出版信息

Wei Sheng Wu Xue Bao. 1999 Oct;39(5):448-53.

PMID:12555527

Abstract

All of the enzymes relating to aniline degradation in C. acidovorans AN3 were inducible ones. When growing on aniline as sole carbon, nitrogen and energy sources, the cells of C. acidovorans AN3 contained aniline dioxygenase, catechol-2, 3-dioxygenase, 2-hydromuconic semialdehyde dehydrogenase, 4-oxalocrotonic acid decarboxylase and 4-hydroxy-2-oxovalerate acid aldolase. The Km and Vmax of aniline dioxygenase were 292 mumol/L and 3.57 mumol.mg-1. min-1, respectively. The Km and Vmax of catechol-2, 3-dioxygenase were 16.4 mumol/L and 15.2 mumol.mg-1.min-1, respectively. According to these results, the degradation metabolism pathway of aniline by C. acidovorans AN3 was hypothesised.

摘要

嗜酸氧化亚铁硫杆菌AN3中所有与苯胺降解相关的酶都是诱导酶。当嗜酸氧化亚铁硫杆菌AN3以苯胺作为唯一碳源、氮源和能源生长时，其细胞中含有苯胺双加氧酶、儿茶酚-2,3-双加氧酶、2-羟基粘康酸半醛脱氢酶、4-氧代巴豆酸脱羧酶和4-羟基-2-氧代戊酸醛缩酶。苯胺双加氧酶的Km和Vmax分别为292 μmol/L和3.57 μmol·mg-1·min-1。儿茶酚-2,3-双加氧酶的Km和Vmax分别为16.4 μmol/L和15.2 μmol·mg-1·min-1。根据这些结果，推测了嗜酸氧化亚铁硫杆菌AN3降解苯胺的代谢途径。