Bazot S, Bois P, Joyeux C, Lebeau T
Equipe Dépollution Biologique des Sols, Université de Haute Alsace, 33, rue de Herrlisheim, 50568, 68008, Colmar Cedex, France.
Biotechnol Lett. 2007 May;29(5):749-54. doi: 10.1007/s10529-007-9316-7. Epub 2007 Feb 27.
Mineralization of diuron has not been previously demonstrated despite the availability of some bacteria to degrade diuron into 3,4-dichloroaniline (3,4-DCA) and others that can mineralize 3,4-DCA. A bacterial co-culture of Arthrobacter sp. N4 and Delftia acidovorans W34, which respectively degraded diuron (20 mg l(-1)) to 3,4-DCA and mineralized 3,4-DCA, were able to mineralize diuron. Total diuron mineralization (20 mg l(-1)) was achieved with free cells in co-culture. When the bacteria were immobilized (either one bacteria or both), the degradation rate was higher. Best results were obtained with free Arthrobacter sp. N4 cells co-cultivated with immobilized cells of D. acidovorans W34 (mineralization of diuron in 96 h, i.e., 0.21 mg l(-1 )h(-1) vs. 0.06 mg l(-1 )h(-1) with free cells in co-culture).
尽管有一些细菌能够将敌草隆降解为3,4-二氯苯胺（3,4-DCA），还有其他细菌能够将3,4-DCA矿化，但此前尚未证明敌草隆的矿化作用。节杆菌属N4菌株和食酸戴尔福特菌W34的细菌共培养物，分别将敌草隆（20 mg l(-1)）降解为3,4-DCA并使3,4-DCA矿化，能够使敌草隆矿化。共培养中的游离细胞实现了敌草隆的完全矿化（20 mg l(-1)）。当细菌固定化（一种细菌或两种细菌）时，降解率更高。将游离的节杆菌属N4菌株细胞与固定化的食酸戴尔福特菌W34细胞共培养，获得了最佳结果（96小时内敌草隆矿化，即0.21 mg l(-1 )h(-1)，而共培养中的游离细胞为0.06 mg l(-1 )h(-1)）。
