Sharma S C, Sharma S, Gulati O P
Department of Pharmacology and Therapeutics, Trinity College, Dublin-2, Ireland.
Phytother Res. 2003 Jan;17(1):66-9. doi: 10.1002/ptr.1240.
Oxygen derived free radicals are now increasingly regarded as a primary force of tissue destruction and also have the ability to release histamine from mast cells. Pycnogenol is an extract of the bark of French maritime pine (Pinus pinaster) containing bioflavonoids with a potent ability to scavenge free radicals. Therefore Pycnogenol was investigated for inhibition of histamine release from rat peritoneal mast cells. In addition, its effects were compared with sodium cromoglycate, a known inhibitor of histamine release from the mast cell. Rat peritoneal mast cells were isolated and purified by differential centrifugation and cells pooled from 3-4 animals were suspended at approximately 10(6) cells/mL buffered salt solution. Histamine release was induced by compound 48/80 or the calcium ionophore A-23187 and estimated from supernatant following extraction and by fluorimetric methods. Pycnogenol produced a concentration dependent inhibition of histamine release induced by the two secretagogues. Its inhibitory effect on mast cell histamine release was favourably comparable to sodium cromoglycate.
氧衍生的自由基现在越来越被视为组织破坏的主要力量，并且还具有从肥大细胞释放组胺的能力。碧萝芷是法国滨海松（Pinus pinaster）树皮的提取物，含有具有强大自由基清除能力的生物类黄酮。因此，对碧萝芷抑制大鼠腹膜肥大细胞组胺释放的作用进行了研究。此外，将其效果与色甘酸钠（一种已知的肥大细胞组胺释放抑制剂）进行了比较。通过差速离心法分离和纯化大鼠腹膜肥大细胞，将来自3 - 4只动物的细胞汇集后，以约10(6)个细胞/mL的缓冲盐溶液悬浮。组胺释放由化合物48/80或钙离子载体A - 23187诱导，并通过提取后的上清液和荧光法进行估算。碧萝芷对两种促分泌剂诱导的组胺释放产生浓度依赖性抑制。其对肥大细胞组胺释放的抑制作用与色甘酸钠相当。
