Department of Anatomy, Chonbuk National University Medical School, Jeonju, Jeonbuk, 561-756, Republic of Korea.
Phytother Res. 2009 Dec;23(12):1691-5. doi: 10.1002/ptr.2812.
IgE-dependent mast cell activation is known to be associated with the allergic diseases. Pycnogenol (PYC) is a standardized extract of the bark of French maritime pine containing bioflavonoids with a potent antioxidant activity. The antiallergic activity of PYC was evaluated using both in vivo and in vitro experimental models. Oral administration of PYC significantly inhibited anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis in rats. In an in vitro study, PYC dose-dependently reduced histamine release from rat peritoneal mast cells (RPMC) triggered by anti-DNP IgE. PYC inhibited the protein expression and secretion of tumor necrosis factor-alpha and interleukin-6 in anti-DNP IgE-stimulated RPMC. Moreover, PYC decreased anti-DNP IgE-induced calcium uptake into RPMC. Furthermore, PYC suppressed nuclear factor-kappa B activation. From these results, the clinical use of PYC in the mast cell-mediated immediate-type allergic diseases is proposed.
已知 IgE 依赖性肥大细胞活化与过敏性疾病有关。碧萝芷（PYC）是法国沿海松树树皮的标准化提取物，含有具有强大抗氧化活性的生物类黄酮。使用体内和体外实验模型评估了 PYC 的抗过敏活性。口服 PYC 可显著抑制大鼠抗二硝基苯（DNP）IgE 介导的被动皮肤过敏反应。在一项体外研究中，PYC 剂量依赖性地减少了抗 DNP IgE 触发的大鼠腹腔肥大细胞（RPMC）中组胺的释放。PYC 抑制了肿瘤坏死因子-α和白细胞介素-6在抗 DNP IgE 刺激的 RPMC 中的蛋白表达和分泌。此外，PYC 减少了抗 DNP IgE 诱导的 RPMC 内钙摄取。此外，PYC 抑制了核因子-κB 的激活。基于这些结果，提出了 PYC 在肥大细胞介导的即刻型过敏性疾病中的临床应用。
