Karacić Visnja, Skender Ljiljana, Brcić Irena, Bagarić Ante
Institute for Medical Research and Occupational Health, Psychiatric Hospital Vrapce, Zagreb, Croatia.
Arh Hig Rada Toksikol. 2002 Sep;53(3):213-20.
This article gives a brief account of a two-year experience with gas chromatography/mass spectrometry methods developed for the analysis of opiates (morphine, codeine, heroin and 6-acetylmorphine), cocaine, methadone, and amphetamines (amphetamine; methamphetamine; 3,4-methylenedioxyamphetamine--MDA, and 3,4-methylenedioxymethamphetamine--MDMA, Ecstasy) in hair. The methods developed were reproducible (RSD = 5.0-16.1%), accurate (85.1-100.6%) and sensitive (LD = 0.05-0.30 ng/mg). They were applied in the analysis of 221 hair samples obtained from young subjects aged 15-25 years, who were suspected of drug abuse. Seventy-seven hair samples were found positive for drugs of abuse. Forty-two subjects were found to have consumed amphetamine, of whom 40 were found MDMA (Ecstasy). Heroin consumption, based on positive 6-acetylmorphine, was found in 26 subjects. Cocaine alone was present in three subjects, two were found cocaine and heroin, two cocaine and methadone, and one cocaine and MDMA. These results could indicate the trend in drug abuse among young people in Croatia.
本文简要介绍了为期两年的气相色谱/质谱法应用经验，该方法用于分析毛发中的阿片类药物（吗啡、可待因、海洛因和6-乙酰吗啡）、可卡因、美沙酮以及苯丙胺类药物（苯丙胺、甲基苯丙胺、3,4-亚甲基二氧基苯丙胺——MDA和3,4-亚甲基二氧基甲基苯丙胺——MDMA，摇头丸）。所开发的方法具有可重复性（相对标准偏差=5.0 - 16.1%）、准确性（85.1 - 100.6%）和灵敏度（检测限=0.05 - 0.30纳克/毫克）。这些方法应用于对221份来自15至25岁年轻受试者的毛发样本进行分析，这些受试者涉嫌药物滥用。77份毛发样本被检测出滥用药物呈阳性。发现42名受试者曾使用苯丙胺，其中40人使用过MDMA（摇头丸）。基于6-乙酰吗啡呈阳性，发现26名受试者曾使用过海洛因。仅3名受试者体内含有可卡因，2名受试者体内同时含有可卡因和海洛因，2名同时含有可卡因和美沙酮，1名同时含有可卡因和MDMA。这些结果可以表明克罗地亚年轻人药物滥用的趋势。
