el-Aziz M M, Ghazy A A, Effat M M
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
J Egypt Soc Parasitol. 2001 Apr;31(1):327-34.
Two hundreds blood samples and the corresponding faecal and/or bile (114 Egyptian buffaloes, 68 native breed cattle and 18 imported cattle) were examined for Fasciola infection. ELISA revealed that 8 (7.00%) buffaloes, 52 (76.50%) native breed cattle and 15 (83.33%) imported cattle were positive. Parasitologically, the results revealed that 38 (33.33%) buffaloes and 18 (26.50%) native breed cattle were positive, while all imported cattle were free from Fasciola. So, ELISA technique using F. hepatica excretory-secretory (E/S) antigens is of diagnostic value for cattle fasciolosis rather than for buffaloes.
对200份血液样本以及相应的粪便和/或胆汁样本（114头埃及水牛、68头本地品种牛和18头进口牛）进行了肝片吸虫感染检测。酶联免疫吸附测定（ELISA）显示，8头（7.00%）水牛、52头（76.50%）本地品种牛和15头（83.33%）进口牛呈阳性。从寄生虫学角度来看，结果显示38头（33.33%）水牛和18头（26.50%）本地品种牛呈阳性，而所有进口牛均未感染肝片吸虫。因此，使用肝片吸虫排泄-分泌（E/S）抗原的ELISA技术对牛肝片吸虫病具有诊断价值，而对水牛则不然。
