利用肝片吸虫排泄分泌抗原酶联免疫吸附测定法对牛片形吸虫病进行免疫诊断。
Immunodiagnosis of bovine fasciolosis using Fasciola hepatica excretory-secretory antigens ELISA.
作者信息
el-Aziz M M, Ghazy A A, Effat M M
机构信息
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
出版信息
J Egypt Soc Parasitol. 2001 Apr;31(1):327-34.
Two hundreds blood samples and the corresponding faecal and/or bile (114 Egyptian buffaloes, 68 native breed cattle and 18 imported cattle) were examined for Fasciola infection. ELISA revealed that 8 (7.00%) buffaloes, 52 (76.50%) native breed cattle and 15 (83.33%) imported cattle were positive. Parasitologically, the results revealed that 38 (33.33%) buffaloes and 18 (26.50%) native breed cattle were positive, while all imported cattle were free from Fasciola. So, ELISA technique using F. hepatica excretory-secretory (E/S) antigens is of diagnostic value for cattle fasciolosis rather than for buffaloes.
对200份血液样本以及相应的粪便和/或胆汁样本（114头埃及水牛、68头本地品种牛和18头进口牛）进行了肝片吸虫感染检测。酶联免疫吸附测定（ELISA）显示，8头（7.00%）水牛、52头（76.50%）本地品种牛和15头（83.33%）进口牛呈阳性。从寄生虫学角度来看，结果显示38头（33.33%）水牛和18头（26.50%）本地品种牛呈阳性，而所有进口牛均未感染肝片吸虫。因此，使用肝片吸虫排泄-分泌（E/S）抗原的ELISA技术对牛肝片吸虫病具有诊断价值，而对水牛则不然。
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