Ikeda Ikuhiro, Tsukahara Satoshi, Watarai Hitoshi
Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Anal Sci. 2003 Jan;19(1):27-31. doi: 10.2116/analsci.19.27.
The dielectrophoretic (DEP) behavior of individual yeast cells (5-7 microm in diameter) in aqueous media was observed in a fabricated planar quadrupole microelectrode with a working area of 100 microm in diameter by an optical microscope. The yeast cells migrated in the radial direction in the working area. The DEP velocity of the cells increased as they approached the electrode. The DEP trajectory of the cells was analyzed with a theoretical equation derived previously, and the dielectrophoretic mobility was determined. The dielectrophoretic mobility was found to be affected by the viability of cells, the conductivity of the medium, and the binding of lectin protein (concanavalin A) to the cell surface. These DEP behaviors were analyzed based on the permittivities and conductivities of the cell interior and wall, and those of the medium.
通过光学显微镜在直径为100微米的预制平面四极微电极中观察了直径为5 - 7微米的单个酵母细胞在水性介质中的介电泳（DEP）行为。酵母细胞在工作区域内沿径向迁移。细胞的DEP速度随着它们靠近电极而增加。用先前推导的理论方程分析了细胞的DEP轨迹，并确定了介电泳迁移率。发现介电泳迁移率受细胞活力、介质电导率以及凝集素蛋白（伴刀豆球蛋白A）与细胞表面结合的影响。基于细胞内部和细胞壁以及介质的介电常数和电导率对这些DEP行为进行了分析。
