Hytönen Vesa P, Laitinen Olli H, Grapputo Alessandro, Kettunen Anu, Savolainen Janne, Kalkkinen Nisse, Marttila Ari T, Nordlund Henri R, Nyholm Thomas K M, Paganelli Giovanni, Kulomaa Markku S
Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35 (YAB), Jyväskylä, FIN-40014, Finland.
Biochem J. 2003 May 15;372(Pt 1):219-25. doi: 10.1042/BJ20021531.
Chicken avidin and bacterial streptavidin are proteins used in a wide variety of applications in the life sciences due to their strong affinity for biotin. A new and promising use for them is in medical pretargeting cancer treatments. However, their pharmacokinetics and immunological properties are not always optimal, thereby limiting their use in these applications. To search for potentially beneficial new candidates, we screened egg white from four different poultry species for avidin. Avidin proteins, isolated from the duck, goose, ostrich and turkey, showed a similar tetrameric structure, similar glycosylation and stability against both temperature and proteolytic activity of proteinase K as chicken avidin. Biotin-binding properties of these avidins, measured using IAsys optical biosensor, were similar to those found in avidin from the chicken. Three of these novel avidins, however, showed different immunological cross-reactivities when compared with chicken avidin. The patient sera responses to duck, goose and ostrich avidins were also lower than those observed for chicken and turkey avidins. Our findings suggest that the use of these proteins offers advantages over chicken avidin and bacterial streptavidin in pretargeting applications.
鸡抗生物素蛋白和细菌链霉抗生物素蛋白是生命科学中广泛应用的蛋白质，因为它们对生物素具有很强的亲和力。它们一种新的、有前景的用途是在医学前靶向癌症治疗中。然而，它们的药代动力学和免疫学特性并不总是最佳的，从而限制了它们在这些应用中的使用。为了寻找潜在有益的新候选物，我们从四种不同家禽物种的蛋清中筛选抗生物素蛋白。从鸭、鹅、鸵鸟和火鸡中分离出的抗生物素蛋白显示出与鸡抗生物素蛋白相似的四聚体结构、相似的糖基化以及对温度和蛋白酶K的蛋白水解活性的稳定性。使用IAsys光学生物传感器测量的这些抗生物素蛋白的生物素结合特性与鸡抗生物素蛋白中的特性相似。然而，与鸡抗生物素蛋白相比，其中三种新型抗生物素蛋白显示出不同的免疫交叉反应性。患者血清对鸭、鹅和鸵鸟抗生物素蛋白的反应也低于鸡和火鸡抗生物素蛋白。我们的研究结果表明，在预靶向应用中，使用这些蛋白质比鸡抗生物素蛋白和细菌链霉抗生物素蛋白具有优势。
