Smits Theo H M, Wick Lukas Y, Harms Hauke, Keel Christoph
ENAC - ISTE - LPE, Swiss Federal Institute of Technology Lausanne (EPFL), Switzerland.
Environ Microbiol. 2003 Feb;5(2):85-91. doi: 10.1046/j.1462-2920.2003.00389.x.
A method for the quantitative analysis of the hydrophobicity of the mycelial mat of filamentous fungi based on contact angle measurements is presented. It was tested for a range of fungi belonging to the classes of basidiomycetes, ascomycetes and deuteromycetes. The measured contact angles of the mycelial mats ranged between hydrophilic (<30 degrees) for the deuteromycetes Fusarium oxysporum Fo47 GUS1 and Trichoderma harzianum P1[pZEGA1] and hydrophobic (>60 degrees) for the ascomycete Cladosporium sp. DSE48.1b and the basidiomycetes Paxillus involutus WSL 37.7, Hebeloma crustiliniforme WSL 6.2, Suillus bovinus WSL 48.1 and Laccaria bicolor WSL 73.1. For some fungi, variations in the hydrophobicity of the mycelium depending on the growth medium, the physiological state and the exposure to water were distinguished.
提出了一种基于接触角测量的丝状真菌菌丝体垫疏水性定量分析方法。该方法针对一系列担子菌纲、子囊菌纲和半知菌类真菌进行了测试。菌丝体垫的测量接触角范围为：半知菌尖孢镰刀菌Fo47 GUS1和哈茨木霉P1[pZEGA1]为亲水性（<30度），子囊菌枝孢菌DSE48.1b以及担子菌卷缘桩菇WSL 37.7、皮壳疣柄牛肝菌WSL 6.2、乳牛肝菌WSL 48.1和双色蜡蘑WSL 73.1为疏水性（>60度）。对于一些真菌，区分了菌丝体疏水性随生长培养基、生理状态和接触水情况的变化。
