噬菌体m13基因5和基因8信息的功能半衰期。
Functional half-lives of bacteriophage m13 gene 5 and gene 8 messages.
作者信息
Lica L, Ray D S
出版信息
J Virol. 1976 Apr;18(1):80-4. doi: 10.1128/JVI.18.1.80-84.1976.
Abstract
The half-lives of the M13 gene 5 and gene 8 messages were determined by measuring the decay in the rate of synthesis of the gene 5 and gene 8 proteins after inhibition of new RNA chain initiations with rifampin. The gene 5 and gene 8 messages decay with half-lives of approximately 2.5 and 5 min, respectively. We found no evidence of a functional M13 message with a half-life as long as that reported for hybridizable mRNA.
摘要
通过用利福平抑制新的RNA链起始后，测量M13基因5和基因8蛋白质合成速率的衰减，来确定M13基因5和基因8信息的半衰期。基因5和基因8信息的衰减半衰期分别约为2.5分钟和5分钟。我们没有发现半衰期与报道的可杂交mRNA一样长的功能性M13信息的证据。
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