McKeown Scott Thomas William, Hyland Paula Lisa, Locke Matthew, Mackenzie Ian Campbell, Irwin Christopher Robert
Oral Science Research Center, School of Dentistry, Queen's University Belfast, Northern Ireland, UK.
Eur J Oral Sci. 2003 Feb;111(1):42-50. doi: 10.1034/j.1600-0722.2003.00002.x.
Keratinocyte growth factor (KGF) and hepatocyte growth factor/scatter factor (SF) are two signalling molecules thought to play important roles in regulating epithelial-mesenchymal interactions. Expression of both factors by fibroblasts in subepithelial connective tissue may play a role in maintaining epithelial integrity in health and in the apical migration of junctional epithelium in periodontitis. The aims of this study were (a) to compare expression levels of KGF and SF by periodontal ligament (PDL) and gingival fibroblasts; and (ii) to determine the effects of interleukin (IL)-1 beta, transforming growth factor (TGF)-beta 1, platelet-derived growth factor (PDGF)-BB and epidermal growth factor (EGF) on KGF/SF expression by these cell populations. Three paired PDL and gingival fibroblast strains were developed. The KGF and SF protein levels were analysed by enzyme-linked immunosorbent assay. Relative levels of KGF and SF mRNA in cytokine-treated cultures were determined using semiquantitative reverse transcriptase polymerase chain reaction. No differences in the levels of KGF and SF produced by PDL and gingival (SOG) populations were found. In both cell types IL-1 beta stimulated KGF and SF expression, while TGF-beta 1 significantly inhibited expression at both the mRNA and protein levels. Epidermal growth factor and PDGF-BB induced differing effects on expression, stimulating SF protein production but inhibiting KGF output in both fibroblast populations. Differences in response to EGF and PDGF were also seen between paired PDL and gingival fibroblasts.
角质形成细胞生长因子（KGF）和肝细胞生长因子/散射因子（SF）是两种被认为在调节上皮-间充质相互作用中起重要作用的信号分子。上皮下结缔组织中的成纤维细胞表达这两种因子，可能在维持健康状态下的上皮完整性以及牙周炎时结合上皮的根尖迁移中发挥作用。本研究的目的是：（a）比较牙周膜（PDL）和成纤维细胞中KGF和SF的表达水平；（ii）确定白细胞介素（IL）-1β、转化生长因子（TGF）-β1、血小板衍生生长因子（PDGF）-BB和表皮生长因子（EGF）对这些细胞群体KGF/SF表达的影响。建立了三对PDL和成纤维细胞系。通过酶联免疫吸附测定法分析KGF和SF蛋白水平。使用半定量逆转录聚合酶链反应测定细胞因子处理培养物中KGF和SF mRNA的相对水平。未发现PDL和牙龈（SOG）群体产生的KGF和SF水平存在差异。在两种细胞类型中，IL-1β均刺激KGF和SF表达，而TGF-β1在mRNA和蛋白质水平均显著抑制表达。表皮生长因子和PDGF-BB对表达产生不同影响，刺激两种成纤维细胞群体中SF蛋白的产生，但抑制KGF的输出。在配对的PDL和成纤维细胞之间也观察到对EGF和PDGF反应的差异。
