Worapamorn W, Tam S P, Li H, Haase H R, Bartold P M
School of Dentistry, Department of Physiology and Pharmacology, The University of Queensland, Brisbane, QLD, Australia.
J Periodontal Res. 2002 Aug;37(4):273-8. doi: 10.1034/j.1600-0765.2002.01610.x.
Cell-surface proteoglycans participate in several biological functions including interactions with a variety of growth factors and cytokines. Regulation of syndecan-1 and -2 gene expression was investigated in human periodontal ligament fibroblasts (PDLF), osteoblasts (OB) and gingival fibroblasts (GF), in response to platelet-derived growth factor (PDGF-BB), transforming growth factor (TGF-beta 1), and interleukin (IL-1 beta) by Northern blot analyses. We also compared the effect of PDGF-BB and TGF-beta 1, separately and in combination, in the prolonged presence of IL-1 beta on the expression of both syndecan genes. The results demonstrated that the three cell lines regulated the expression of syndecan-1 and -2 in response to growth factors and cytokines in different manners. These cell lines increased syndecan-1 mRNA levels in response to either PDGF-BB or TGF-beta 1 and decreased levels in response to IL-1 beta. The effect of IL-1 beta on syndecan-1 mRNA synthesis was partially reversed after adding PDGF-BB and TGF-beta 1, separately or in combination, in the presence of IL-1 beta. In contrast, syndecan-2 mRNA level was markedly upregulated in response to either TGF-beta 1 or IL-1 beta in OB when compared with the other two cell lines. However, the stimulatory effect of TGF-beta 1 on syndecan-2 mRNA production in OB was abolished in the prolonged presence of IL-1 beta. These findings lend support to the notion that syndecan-1 and syndecan-2 have distinct functions which correlate with their source and functions within the periodontium.
细胞表面蛋白聚糖参与多种生物学功能，包括与多种生长因子和细胞因子的相互作用。通过Northern印迹分析，研究了人牙周膜成纤维细胞（PDLF）、成骨细胞（OB）和牙龈成纤维细胞（GF）中syndecan-1和-2基因表达对血小板衍生生长因子（PDGF-BB）、转化生长因子（TGF-β1）和白细胞介素（IL-1β）的反应。我们还比较了在IL-1β长期存在的情况下，PDGF-BB和TGF-β1单独及联合作用对两种syndecan基因表达的影响。结果表明，这三种细胞系以不同方式调节syndecan-1和-2对生长因子和细胞因子的反应。这些细胞系对PDGF-BB或TGF-β1反应时syndecan-1 mRNA水平升高，对IL-1β反应时水平降低。在IL-1β存在的情况下分别或联合添加PDGF-BB和TGF-β1后，IL-1β对syndecan-1 mRNA合成的影响部分被逆转。相比之下，与其他两种细胞系相比，OB细胞中syndecan-2 mRNA水平在对TGF-β1或IL-1β反应时显著上调。然而，在IL-1β长期存在的情况下，TGF-β1对OB细胞中syndecan-2 mRNA产生的刺激作用被消除。这些发现支持了syndecan-1和syndecan-2具有与其在牙周组织中的来源和功能相关的不同功能这一观点。
