Zanoni J N, Buttow N C, Bazotte R B, Miranda Neto M H
Department of Morphophysiological Sciences (DCM/UEM), State University of Maringá, 87020-900 Maringá, PR, Brazil.
Auton Neurosci. 2003 Feb 28;104(1):32-8. doi: 10.1016/S1566-0702(02)00266-7.
We investigated the effect of the ascorbic acid on the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d)-stained and myosin-V myenteric neurons in the ileum of chronically diabetic rats. The study was performed 4 months after inducing experimental diabetes with streptozotocin. Diabetic rats showed increased (p<0.05) glycaemia and glycated haemoglobin. Three groups were compared, i.e., nondiabetic rats, diabetic rats and diabetic rats treated with ascorbic acid. Myosin-V immunohistochemistry and NADPH-d histochemistry were employed. We investigated the areas of 500 cell bodies of myosin-V neurons and of 500 NADPH-d-stained neurons from all groups. The quantitative analysis was performed by using an area of 8.96 mm(2) from each ileum. The two groups of diabetic rats and diabetic rats treated with ascorbic acid showed reduction in the number and an increased area of the myosin-V-immunostained myenteric neurons. In addition, we observed increased relative proportion of NADPH-d-stained neurons in diabetic rats and diabetic rats treated with ascorbic acid. However, the area of these neurons in the diabetic rats group was larger than those evidenced in the nondiabetic rats and diabetic rats treated with ascorbic acid.
我们研究了抗坏血酸对慢性糖尿病大鼠回肠中烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）染色的和肌球蛋白-V肌间神经元的影响。该研究在链脲佐菌素诱导实验性糖尿病4个月后进行。糖尿病大鼠的血糖和糖化血红蛋白升高（p<0.05）。比较了三组，即非糖尿病大鼠、糖尿病大鼠和用抗坏血酸治疗的糖尿病大鼠。采用肌球蛋白-V免疫组织化学和NADPH-d组织化学方法。我们研究了所有组中500个肌球蛋白-V神经元细胞体和500个NADPH-d染色神经元的面积。通过使用来自每个回肠的8.96平方毫米的面积进行定量分析。两组糖尿病大鼠和用抗坏血酸治疗的糖尿病大鼠显示，肌球蛋白-V免疫染色的肌间神经元数量减少，面积增加。此外，我们观察到糖尿病大鼠和用抗坏血酸治疗的糖尿病大鼠中NADPH-d染色神经元的相对比例增加。然而，糖尿病大鼠组中这些神经元的面积大于非糖尿病大鼠和用抗坏血酸治疗的糖尿病大鼠中的面积。
