Department of Morphological Sciences, Statal University of Maringá, PR, Brazil.
Histol Histopathol. 2012 Jan;27(1):123-32. doi: 10.14670/HH-27.123.
We examined the effects of ascorbic acid supplementation on myosin-V, calcitonin gene-related peptide (CGRP) and vasoactive intestinal polypeptide (VIP) immunoractivities in the myenteric neurons in aging rats. Male rats were divided into groups: young 90-day-old rats (E90), 345-day-old control rats (E345), 428-day-old control rats (E428), 90- to 345-day-old rats treated with ascorbic acid (1 g/L) (EA345), and 90- to 428-day-old rats treated with ascorbic acid (1g/L) (EA428). The quantitative results showed that aging reduced the number of myosin-V-immunoreactive neurons compared with young animals (E90). Ascorbic acid supplementation in the EA345 and EA428 groups increased the average area of myosin-V neurons by 24.6% and 24.1% compared with the E345 and E428 groups, respectively. When all groups were compared, we observed significant differences for the CGRP- and VIP-immunoractive varicosities of nerve fibers from myenteric neurons. Ascorbic acid supplementation had a neurotrophic effect on all neurons studied, suggesting a neuroprotective role.
我们研究了抗坏血酸补充对衰老大鼠肌间神经元肌球蛋白-V、降钙素基因相关肽（CGRP）和血管活性肠肽（VIP）免疫反应的影响。雄性大鼠分为以下几组：90 日龄的年轻大鼠（E90）、345 日龄的对照组大鼠（E345）、428 日龄的对照组大鼠（E428）、用抗坏血酸（1g/L）处理的 90 至 345 日龄大鼠（EA345）和用抗坏血酸（1g/L）处理的 90 至 428 日龄大鼠（EA428）。定量结果表明，与年轻动物（E90）相比，衰老减少了肌球蛋白-V 免疫反应神经元的数量。与 E345 和 E428 组相比，EA345 和 EA428 组补充抗坏血酸分别使肌球蛋白-V 神经元的平均面积增加了 24.6%和 24.1%。当所有组进行比较时，我们观察到肌间神经元神经纤维的 CGRP 和 VIP 免疫反应性轴突之间存在显著差异。抗坏血酸补充对所有研究神经元均具有神经营养作用，提示其具有神经保护作用。
