采用离子对高效液相色谱法快速测定人血浆中的二甲双胍。
Rapid determination of metformin in human plasma using ion-pair HPLC.
作者信息
Zarghi A, Foroutan S M, Shafaati A, Khoddam A
机构信息
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
J Pharm Biomed Anal. 2003 Feb 5;31(1):197-200. doi: 10.1016/s0731-7085(02)00608-8.
A rapid, simple and sensitive ion-pair HPLC method has been developed for quantification of metformin in plasma. The assay enables the measurement of metformin for therapeutic drug monitoring with a minimum detectable limit of 20 ng/ml. The method involves simple, one-step extraction procedure and analytical recovery was complete. The separation was performed on an analytical 150 x 4.6 mm i.d. mubondapak C(18) column. The wavelength was set at 235 nm. The mobile phase was 40% acetonitrile, 0.01 M sodium dodecyl sulphate, 0.01 M sodium dihydrogen phosphate, and distilled water to 100%, adjusted to pH 5.1 at a flow rate of 1.5 ml/min. The calibration curve was linear over the concentration range 0.2-2.5 microg/ml. The coefficients of variation for inter-day and intra-day assay were within the range of clinical usefulness.
已开发出一种快速、简单且灵敏的离子对高效液相色谱法用于定量测定血浆中的二甲双胍。该测定法能够测定二甲双胍以进行治疗药物监测，最低检测限为20 ng/ml。该方法涉及简单的一步萃取程序，且分析回收率完全。分离在一根150×4.6 mm内径的分析型mubondapak C(18)柱上进行。波长设定为235 nm。流动相为40%乙腈、0.01 M十二烷基硫酸钠、0.01 M磷酸二氢钠，加蒸馏水至100%，调节pH值至5.1，流速为1.5 ml/min。校准曲线在0.2 - 2.5 μg/ml的浓度范围内呈线性。日间和日内测定的变异系数在临床可用范围内。
Zotero 插件