从鸟博德特氏菌ATCC 5086分离的O链多糖的结构表征：同一主题的变体(1)
Structural characterization of the O-chain polysaccharide isolated from Bordetella avium ATCC 5086: variation on a theme(1).
作者信息
Larocque Suzon, Brisson Jean-Robert, Thérisod Hélène, Perry Malcolm B, Caroff Martine
机构信息
Institute for Biological Sciences, NRC, Ottawa, ON, Canada.
出版信息
FEBS Lett. 2003 Jan 30;535(1-3):11-6. doi: 10.1016/s0014-5793(02)03843-7.
The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI studies showed the O-chain to be a polymer composed of 1,4-linked 2-acetamidino-3-[3-hydroxybutanamido]-2,3-dideoxy-beta-D-glucopyranosyluronic acid residues.
禽博德特氏菌的O链多糖（O-PS）通过温和酸水解从脂多糖中分离出来，以去除脂质A，随后进行氢氟酸水解以去除脂多糖核心寡糖，留下残余的O-PS用于结构分析。高分辨率的¹H和¹³C核磁共振以及基质辅助激光解吸电离研究表明，O链是一种由1,4-连接的2-脒基-3-[3-羟基丁酰胺基]-2,3-二脱氧-β-D-吡喃葡萄糖醛酸残基组成的聚合物。
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