基质辅助激光解吸/电离质谱法分析碳水化合物和糖缀合物：2003 - 2004年最新进展
Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2003-2004.
作者信息
Harvey David J
机构信息
Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK.
出版信息
Mass Spectrom Rev. 2009 Mar-Apr;28(2):273-361. doi: 10.1002/mas.20192.
Abstract
This review is the third update of the original review, published in 1999, on the application of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings the topic to the end of 2004. Both fundamental studies and applications are covered. The main topics include methodological developments, matrices, fragmentation of carbohydrates and applications to large polymeric carbohydrates from plants, glycans from glycoproteins and those from various glycolipids. Other topics include the use of MALDI MS to study enzymes related to carbohydrate biosynthesis and degradation, its use in industrial processes, particularly biopharmaceuticals and its use to monitor products of chemical synthesis where glycodendrimers and carbohydrate-protein complexes are highlighted.
摘要
本综述是1999年发表的关于基质辅助激光解吸/电离（MALDI）质谱在碳水化合物和糖缀合物分析中的应用的原始综述的第三次更新，涵盖了截至2004年底的相关内容。既有基础研究也有应用研究。主要主题包括方法学进展、基质、碳水化合物的碎片化以及在植物来源的大型聚合碳水化合物、糖蛋白聚糖和各种糖脂聚糖分析中的应用。其他主题包括利用MALDI MS研究与碳水化合物生物合成和降解相关的酶，其在工业过程中的应用，特别是生物制药领域，以及用于监测化学合成产物，其中突出了糖树枝状大分子和碳水化合物 - 蛋白质复合物。
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