Cole Alexander M, Liao Hsiang-I, Ganz Tomas, Yang Otto O
Department of Medicine, Division of Pulmonary and Critical Care Medicine, UCLA School of Medicine, 10833 Le Conte Ave., Room CHS 37-055, Los Angeles, CA 90095-1690, USA.
FEBS Lett. 2003 Jan 30;535(1-3):195-9. doi: 10.1016/s0014-5793(02)03860-7.
Recent reports have highlighted the anti-HIV-1 activities of defensins, whose structure and charge resemble portions of the HIV-1 transmembrane envelope glycoprotein gp41. The current report explores the obverse, whether peptides derived from HIV-1 envelope glycoproteins can exert antimicrobial activity. Fifteen-residue peptides spanning the entire sequence of HIV-1(MN) gp120 and gp41 were subjected to radial diffusion assays against laboratory strains of Escherichia coli and Listeria monocytogenes. Twenty-four active peptides corresponded predominantly to membrane-active domains of gp120 and gp41. Several peptides retained significant activity in higher ionic conditions and may serve as templates for the development of novel peptide antibiotics. The strategies employed herein could uncover additional antimicrobial peptides from envelope proteins of other lytic viruses.
近期报告强调了防御素的抗HIV-1活性，其结构和电荷与HIV-1跨膜包膜糖蛋白gp41的部分相似。本报告探讨相反的情况，即源自HIV-1包膜糖蛋白的肽是否能发挥抗菌活性。针对HIV-1（MN）gp120和gp41的整个序列的15个残基肽，对大肠杆菌和单核细胞增生李斯特菌的实验室菌株进行了径向扩散试验。24种活性肽主要对应于gp120和gp41的膜活性结构域。几种肽在较高离子条件下仍保留显著活性，可作为新型肽类抗生素开发的模板。本文采用的策略可能会从其他裂解病毒的包膜蛋白中发现更多抗菌肽。
