Yuan Yuhua, Bi Changhao, Li Ju, Wang Xueqian, Geng Yunqi, Chen Qimin
College of Life Sciences, Nankai University, 300071 Tianjin, China.
Chin Sci Bull. 2004;49(7):668-671. doi: 10.1007/BF03184262.
The failed attempt to express HIV- in led to the investigation of HIV- segments, which is responsible for the toxicity to cells. A series of deletion mutants containing different regions of gene were constructed and expressed in BL21(DE3) strain. After IPTG induction, the high mortality of host bacteria was observed in host bacteria carrying the deletion mutants of gene except for those transformed with pET-H; coordinately, the mRNA transcripts of the was rapidly decreased; and the release of [H]uridine increased upon induction. All these data suggested that GP41 protein has a cytotoxic effect on, and it is the cytotoxicity of the gene product that contributes to the high mortality when expressed in.
表达HIV-的失败尝试导致了对HIV-片段的研究，该片段对细胞具有毒性。构建了一系列包含基因不同区域的缺失突变体，并在BL21(DE3)菌株中表达。IPTG诱导后，除了用pET-H转化的宿主细菌外，携带基因缺失突变体的宿主细菌中观察到宿主细菌的高死亡率；相应地，的mRNA转录本迅速减少；诱导后[H]尿苷的释放增加。所有这些数据表明，GP41蛋白对具有细胞毒性作用，并且基因产物的细胞毒性是其在中表达时导致高死亡率的原因。
