Flaherty J O, Barrett E J, Bradley D P, Headon D R
Biochim Biophys Acta. 1975 Aug 20;401(2):177-83. doi: 10.1016/0005-2736(75)90302-8.
Basal ATPase is readily separated from the Ca2+-ATPase of the sarcoplasmic reticulum. The median density distributions of cholesterol and basal ATPase activities are almost identical. Digitonin has been successfully employed in determining the association of cholesterol with specific vesicles in rat liver microsomal preparations. Treatment of rabbit skeletal muscle microsomal preparations with digitonin alters the density distribution patterns of basal ATPase activity and cholesterol in an identical fashion. Protein distribution displays a less marked change in median density. Enzymic activity associated with calcium transport, measured under differing conditions, is largely unaffected. It is concluded that cholesterol and basal ATPase activity are associated with a distinct group of rabbit skeletal muscle microsomal particles.
肌浆网的基础ATP酶很容易与Ca2+-ATP酶分离。胆固醇和基础ATP酶活性的中位密度分布几乎相同。洋地黄皂苷已成功用于测定大鼠肝微粒体制剂中胆固醇与特定囊泡的关联。用洋地黄皂苷处理兔骨骼肌微粒体制剂，会以相同方式改变基础ATP酶活性和胆固醇的密度分布模式。蛋白质分布在中位密度上的变化不太明显。在不同条件下测量的与钙转运相关的酶活性基本不受影响。结论是胆固醇和基础ATP酶活性与兔骨骼肌微粒体的一组特定颗粒相关。
