Barrett E J, Ryan N M, Headon D R
Biochem J. 1980 May 15;188(2):569-72. doi: 10.1042/bj1880569.
The influence of homogenization times on the presence of constituents in the microsomal fraction of skeletal muscle was investigated. Membranes having Ca2+-activated ATPase activity have a fragmentation pattern distinct from that of membranes displaying Ca2+-independent or basal ATPase activity. These latter membranes were found in highest specific concentration in the microsomal fraction prepared from homogenates subjected to short periods of homogenization. 5'-Nucleotidase (EC 3.1.3.5) activity paralleled that of basal ATPase on short periods of homogenization, as also did the specific concentration of cholesterol. Longer periods of homogenization led to a decrease in the specific activity of basal atpase, which reached its lowest value at 120s of homogenization, whereas the specific activity of 5'-nucleotidase and the specific concentration of cholesterol decreased initially in a similar manner to basal ATPase, but both increased substantially after the longest period of homogenization.
研究了匀浆时间对骨骼肌微粒体部分成分存在情况的影响。具有Ca2+激活ATP酶活性的膜具有与显示Ca2+非依赖性或基础ATP酶活性的膜不同的破碎模式。在由短时间匀浆的匀浆物制备的微粒体部分中，发现后一种膜的比浓度最高。在短时间匀浆时，5'-核苷酸酶（EC 3.1.3.5）活性与基础ATP酶活性平行，胆固醇的比浓度也是如此。较长时间的匀浆导致基础ATP酶的比活性降低，在匀浆120秒时达到最低值，而5'-核苷酸酶的比活性和胆固醇的比浓度最初与基础ATP酶以类似方式降低，但在最长匀浆时间后两者均大幅增加。
