Zhao Ping, Wang Hong-Wei, Lu Yang, Qi Zhong-Tian
Department of Microbiology, Second Military Medical University, Shanghai 200433, China.
Sheng Wu Gong Cheng Xue Bao. 2002 Sep;18(5):601-4.
The enhanced green fluorescent protein (EGFP) expression plasmid was transformed into an attenuated AroA- autotrophic mutant of Salmonella typhimurium SL7207, the resultant bacteria was administered orally to BALB/c mice. EGFP expressed in spleen cells was detected by flow cytometry. A DNA vaccine encoding HBV large envelope protein was immunized BALB/c mice by oral delivery through SL7207 or by direct intramuscular injection. The serum antibodies, T lymphocyte proliferative response and cytotoxic T lymphocyte response of mice were detected. The results showed that both DNA immunization methods could induce cellular and humoral immune responses, whereas oral vaccination elicited stronger immune responses than intramuscular vaccination did. Therefor, oral administration with HBV DNA vaccine using attenuated Salmonella may be a simple and effective method for the therapy of hepatitis B.
将增强型绿色荧光蛋白（EGFP）表达质粒转化到鼠伤寒沙门氏菌SL7207的aroA自养减毒突变体中，将所得细菌经口给予BALB/c小鼠。通过流式细胞术检测脾细胞中表达的EGFP。编码乙肝病毒大包膜蛋白的DNA疫苗通过SL7207经口递送或直接肌肉注射免疫BALB/c小鼠。检测小鼠的血清抗体、T淋巴细胞增殖反应和细胞毒性T淋巴细胞反应。结果表明，两种DNA免疫方法均可诱导细胞免疫和体液免疫反应，而口服疫苗接种比肌肉注射疫苗接种引发更强的免疫反应。因此，使用减毒沙门氏菌口服给予乙肝病毒DNA疫苗可能是治疗乙型肝炎的一种简单有效的方法。
