Si Yan-Mei, Hao Bing-Tao, Li Yi, Wang Ying-Tai, Zhu Wen-Yu, Yang Yan-Li, Liao Shi-Xiu, Huang Fei-Fei, Wang Zhao-Cai
Medical Genetic Institute of Henan Province, People's Hospital of Henan Province, Zhengzhou, 450003, China.
Yi Chuan Xue Bao. 2002 Oct;29(10):854-9.
To investigate the allele frequencies of six short tandem repeats (STR) loci: F13A1, F13B, D8S1179, CSF1PO, D5S818 and TPOX in Han population in Henan province, DNA was extracted with chelex from EDTA-blood samples of the unrelated individuals in Henan province and amplified with PCR technique. The PCR product was analyzed with the undenatured PAGE vertical electrophoresis and silverstain. The results were obtained through genetical analysis. The authors got the number of allele of six loci, Three alleles of F13A1 could be detected in this population. The F13A1 * 7 is the most common allele with a frequency of 45.2%, followed by F13A1 * 5 and F13A1 * 4 respectively. F13B had four alleles among which F13B * 10 has the highest frequency, followed by F13B * 9, F13B * 8, F13B * 11. The 8 alleles of D8S1179 in the order from high to low frequency is D8S1179 * 14, * 15, * 12, * 11, * 10. And the most common frequencies of alleles of CSF1PO, D5S818, TPOX ore CSF1PO * 12, D5S818 * 11, TPOX * 8, respectively followed by the allele of * 11, * 14, * 10, * 13 in CSF1PO locus, and * 12, * 13, * 10, * 9, * 14, * 8, * 15, * 7 in D5S818 locus, * 11, * 9, * 12, * 10 in TPOX locus. The heterozygosities of the six loci were 0.62, 0.46, 0.83, 0.59, 0.78 and 0.65; the discrimination powers were 0.78, 0.66, 0.95, 0.79, 0.92 and 0.82. The heterozygosities of the six loci are high and the frequencies are in good agreement with Hardy-Weinberg equilibrium. The six loci are good as genetic markers. We can use these loci in dividualidentification and partenity test in forensic area.
为研究河南汉族人群中6个短串联重复序列（STR）基因座：F13A1、F13B、D8S1179、CSF1PO、D5S818和TPOX的等位基因频率，采用螯合树脂法从河南省无关个体的EDTA抗凝血样中提取DNA，并用PCR技术进行扩增。PCR产物经非变性聚丙烯酰胺凝胶垂直电泳和银染分析。通过遗传学分析得出结果。作者得到了6个基因座的等位基因数，该人群中可检测到F13A1的3个等位基因。F13A1 * 7是最常见的等位基因，频率为45.2%，其次分别是F13A1 * 5和F13A1 * 4。F13B有4个等位基因，其中F13B * 10频率最高，其次是F13B * 9、F13B * 8、F13B * 11。D8S1179的8个等位基因按频率从高到低依次为D8S1179 * 14、* 15、* 12、* 11、* 10。CSF1PO、D5S818、TPOX最常见的等位基因分别是CSF1PO * 12、D5S818 * 11、TPOX * 8，其次是CSF1PO基因座的* 11、* 14、* 10、* 13等位基因，D5S818基因座的* 12、* 13、* 10、* 9、* 14、* 8、* 15、* 7等位基因，TPOX基因座的* 11、* 9、* 12、* 10等位基因。6个基因座的杂合度分别为0.62、0.46、0.83、0.59、0.78和0.65；鉴别力分别为0.78、0.66、0.95、0.79、0.92和0.82。6个基因座的杂合度较高，频率符合哈迪-温伯格平衡。这6个基因座可作为良好的遗传标记。可将这些基因座用于法医学领域的个体识别和亲子鉴定。
