Deng Pingjian, Zhao Jin, Liu Jianjun, Fang Shisong
Shenzhen Hygiene and Epidemics Station, Shenzhen 518020, China.
Wei Sheng Yan Jiu. 2002 Feb;31(1):37-40.
Nucleotide-based amplification method is an important system for the identification of genomic modified foods (GMF). Roundup Ready Soybeans (Monsanto company), Bt 176 GM maize (Novartis/Ciba-Geigy company) and Cecropin D capsicum was used as material to search for the feasibility of investigating the safety of GMF by PCR method. Primers specific for inserted genes and crop endogenous genes in Roundup Ready Soybeans, Bt 176 maize and Cecropin D capsicum were applied. The discrimination system for GM soybeans, GM maize and GM capsicum from the counterpart of non-GM products and the detection system for correlating marker genes and transgenes are established. The method was easy and fast, and the corresponding results fixed the standard or declared data.
基于核苷酸的扩增方法是鉴定转基因食品(GMF)的重要系统。以抗农达大豆(孟山都公司)、Bt 176转基因玉米(诺华/汽巴-嘉基公司)和天蚕素D辣椒为材料,探寻用聚合酶链反应(PCR)法研究转基因食品安全的可行性。应用了针对抗农达大豆、Bt 176玉米和天蚕素D辣椒中插入基因和作物内源基因的特异性引物。建立了区分转基因大豆、转基因玉米和转基因辣椒与非转基因对应产品的鉴别系统以及相关标记基因和转基因的检测系统。该方法简便快捷,相应结果确定了标准或申报数据。