Chernykh E R, Sakhno L V, Khonina M A, Tikhonova M A, Kozhevnikov V S, Nikonov S D, Zhdanov O A, Ostanin A A
Probl Tuberk. 2002(7):43-8.
T-cell apoptosis and anergy as possible causes of impaired antigen specific responses and their subpopulation targets in patients with pulmonary tuberculosis were investigated. A decrease in PPD-stimulated proliferative responses were revealed in 43% of the examinees. The impaired PPD response was shown to be associated with both increased lymphocytic apoptosis and the arrest of cell cycle progression. CD4 and CHD8 T cells underwent apoptosis in PPD-stimulated cultures. Whereas a moderate apoptosis of CD4 cell could occur in PPD-reactive patients, accelerated apoptosis of CD8 cells developed only in PPD-unresponsive group. Both T-cell subpopulations displayed a decreased count of cells in S,G2/M phases of a cell cycle. Similar to apoptosis, the anergy of CD8 T cells was typical of PPD-unresponsive patients. Elevated apoptosis and anergy of CD4 and CD8 T cells in vitro were accompanied by a decline in the proportion of T cells and their subpopulations in patients with impaired PPD responses.
研究了T细胞凋亡和无反应性作为肺结核患者抗原特异性反应受损及其亚群靶点的可能原因。43%的受检者显示PPD刺激的增殖反应降低。PPD反应受损与淋巴细胞凋亡增加和细胞周期进程停滞均有关。在PPD刺激的培养物中，CD4和CHD8 T细胞发生凋亡。虽然PPD反应性患者的CD4细胞可能发生中度凋亡，但仅在PPD无反应组中出现CD8细胞加速凋亡。两个T细胞亚群在细胞周期的S期、G2/M期细胞计数均减少。与凋亡相似，CD8 T细胞无反应性是PPD无反应患者的典型特征。体外CD4和CD8 T细胞凋亡增加和无反应性，伴随着PPD反应受损患者中T细胞及其亚群比例的下降。
