Sakhno L V, Tikhonova M A, Kurganova E V, Shevela E Ia, Nikonov S D, Zhdanov O A, Mostovaia G V, Ostanin A A, Chernykh E R
Probl Tuberk Bolezn Legk. 2004(5):23-8.
The phenotypic and functional properties of T cells were evaluated in patients with tuberculin anergy and a possible role of anergic T cells in the development of immune deficiency in pulmonary tuberculosis (PT) was studied. The profound decrease (more than 50%) depressed T-cell proliferation in PPD-stimulated cultures was shown to be recorded in 44% (59/134) patients with PT. PPD hyporesponsiveness was associated with the low proliferation of anti-CD3 and SEB-stimulated proliferation of a healthy donor's mononuclear cells evidencing the enlargement of anergic T cells with a suppressive activity in PT. A PPD-stimulated response in healthy donors was under the negative control of CG25-positive cells. In the PPD-anergic patients, there was a significant increase in CD4+CD25+ T cells that were inversely correlated with the PPD-induced proliferative response. The development of tuberculin anergy was more pronounced in patients with drug resistance. The intensity of a PPD-stimulated response in patients with tuberculin anergy may be restored in the presence of exogenous interleukin-2.
对结核菌素无反应患者的T细胞表型和功能特性进行了评估，并研究了无反应性T细胞在肺结核（PT）免疫缺陷发展中的可能作用。结果显示，44%（59/134）的PT患者在PPD刺激培养物中出现T细胞增殖显著下降（超过50%）。PPD低反应性与抗CD3刺激及健康供体单核细胞的SEB刺激增殖的低增殖相关，这表明PT中具有抑制活性的无反应性T细胞增多。健康供体中PPD刺激反应受CG25阳性细胞的负调控。在PPD无反应患者中，CD4+CD25+T细胞显著增加，且与PPD诱导的增殖反应呈负相关。结核菌素无反应在耐药患者中更为明显。在外源性白细胞介素-2存在的情况下，结核菌素无反应患者中PPD刺激反应的强度可能恢复。
