Kadalmani B, Girija R, Faridha A, Akbarsha M A
Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India.
Indian J Exp Biol. 2002 Jan;40(1):40-4.
Carbendazim (MBC), a widely used fungicide, is toxic to male reproductive mechanisms. Various cellular targets in the testis for MBC action are being understood only recently and still more targets have been conceived. The present study was aimed at finding such newer targets. Male rats were administered through oral route a single dose of carbendazim (400 mg/kg) and the testis was studied adopting routine histological technique. It has been observed that pachytene spermatocytes could also be targets for MBC action in the testis. The study also reports selective loss of step 14 spermatids, asynchrony of the stages in the spermatogenic cycle and development of Sertoli cell fibrosis of the seminiferous tubules of carbendazim-treated rats. From the different kinds of responses seen in the seminiferous tubules in the same testis to MBC, particularly in the Sertoli cell, MBC action in the testis appears dependent on the stage in the spermatogenic cycle at first exposure.
多菌灵（MBC）是一种广泛使用的杀菌剂，对雄性生殖机制有毒性。睾丸中多菌灵作用的各种细胞靶点直到最近才被了解，并且仍有更多靶点被设想出来。本研究旨在寻找此类新靶点。通过口服途径给雄性大鼠单剂量施用多菌灵（400毫克/千克），并采用常规组织学技术对睾丸进行研究。据观察，粗线期精母细胞也可能是睾丸中多菌灵作用的靶点。该研究还报告了14期精子细胞的选择性丢失、生精周期各阶段的不同步以及多菌灵处理大鼠曲细精管支持细胞纤维化的发展。从同一睾丸的曲细精管中观察到的对多菌灵的不同反应类型，特别是在支持细胞中，睾丸中多菌灵的作用似乎首先取决于首次接触时生精周期的阶段。
