Nakai M, Hess R A, Netsu J, Nasu T
Department of Veterinary Anatomy, Miyazaki University, Japan.
J Androl. 1995 Sep-Oct;16(5):410-6.
Carbendazim (methyl 2-benzimidazole carbamate; MBC) has been reported to disrupt the microtubules of Sertoli cells in the rat, suggesting that the cells exposed to carbendazim would fail to maintain their shape due to the loss of their cytoskeleton. The present study was performed to determine whether exposure to carbendazim induced Sertoli cell deformation. For this purpose, changes in the shape and position of Sertoli cell nuclei, that appear to be associated with changes in the shape of the cells themselves, were observed in stage VI-VII seminiferous tubules of rat testis at 3 hours following treatment. Light microscopy and computer-aided three-dimensional reconstruction were also performed to survey the outline of the body. In the controls, parallel-positioned nuclei (nuclei with their longer axis parallel to the basement membrane), perpendicular-positioned nuclei (nuclei with their longer axis perpendicular to the basement membrane), and round-shaped nuclei (nuclei showing a round or regular triangular profile) accounted for approximately 52%, 35%, and 13% of the total, respectively. In Sertoli cells showing carbendazim-induced sloughing of germ cells, however, parallel-positioned nuclei accounted for 21% and the round-shaped nuclei for 51% of the total. Approximately 69% of perpendicular-positioned nuclei were located in the basal part of the cells in the controls, compared with 95% in tubules showing carbendazim-induced sloughing. These changes in individual parameters were significantly different (alpha < 0.05). Light microscopy and computer-aided reconstruction indicated that the body region of Sertoli cells in the control testis was columnar in shape, whereas that of Sertoli cells that showed sloughing was conical. These findings suggest that Sertoli cell cytoplasm, following carbendazim-induced sloughing, moves toward the base of the cellular axis.
据报道，多菌灵（2-苯并咪唑氨基甲酸甲酯；MBC）会破坏大鼠睾丸支持细胞的微管，这表明暴露于多菌灵的细胞会因细胞骨架的丧失而无法维持其形状。本研究旨在确定暴露于多菌灵是否会诱导支持细胞变形。为此，在处理后3小时观察大鼠睾丸VI-VII期生精小管中支持细胞核的形状和位置变化，这些变化似乎与细胞自身形状的变化相关。还进行了光学显微镜和计算机辅助三维重建以观察细胞体轮廓。在对照组中，平行排列的细胞核（长轴平行于基底膜的细胞核）、垂直排列的细胞核（长轴垂直于基底膜的细胞核）和圆形细胞核（呈圆形或规则三角形轮廓的细胞核）分别占总数的约52%、35%和13%。然而，在显示多菌灵诱导生殖细胞脱落的支持细胞中，平行排列的细胞核占总数的21%，圆形细胞核占51%。在对照组中，约69%的垂直排列细胞核位于细胞基部，而在显示多菌灵诱导脱落的小管中这一比例为95%。这些个体参数的变化有显著差异（α<0.05）。光学显微镜和计算机辅助重建表明，对照睾丸中支持细胞的细胞体区域呈柱状，而显示脱落的支持细胞的细胞体区域呈锥形。这些发现表明，多菌灵诱导脱落后，支持细胞的细胞质向细胞轴基部移动。
