Bejarano Pablo A, Mousavi Fathema
Department of Pathology, University of Miami School of Medicine, Jackson Memorial Hospital, Miami, FL 33136, USA.
Arch Pathol Lab Med. 2003 Feb;127(2):193-5. doi: 10.5858/2003-127-193-IASOCT.
The immunohistochemical identification of thyroid transcription factor-1 (TTF-1) is regarded as the presence of a nuclear pattern of staining and is used to identify tumors of thyroid or pulmonary origin. Although there have been reports of cytoplasmic expression of TTF-1, the significance of this pattern has not been studied in detail.
To determine the incidence at which cytoplasmic immunostaining for TTF-1 occurs and to analyze the diagnostic value of this pattern of immunoreactivity.
Histologic sections of 361 consecutive cases of neoplasms stained for TTF-1 were reviewed, and those showing cytoplasmic staining in the tumor cells or in nonneoplastic cells were selected. Clinical correlation was obtained on the latter cases regarding the origin of the tumor.
An immunohistochemistry laboratory in a tertiary-care institution.
The 361 tumors were obtained from 29 organ sites and corresponded to primary and metastatic neoplasms. Twenty-three (6.3%) tumors showed cytoplasmic staining for TTF-1. In 13 of these, the primary site of origin was established with certainty: 7 were lung carcinomas (3 primary lung adenocarcinomas, 1 primary large cell carcinoma, 1 metastatic small cell carcinoma to the liver, 1 metastatic adenocarcinoma to a neck lymph node, and 1 metastatic adenocarcinoma to thigh soft tissue), 3 colonic adenocarcinomas (2 metastases to vertebrae and 1 to lung), 1 metastatic breast ductal adenocarcinoma to femur, 1 metastatic laryngeal squamous cell carcinoma to liver, and 1 meningioma involving the orbit bone. There were 3 lung carcinomas with concomitant nuclear immunostaining. Nonneoplastic liver tissue was the most frequent nontumoral tissue in which the cytoplasm stained with TTF-1 antibody.
Occasional cytoplasmic staining for TTF-1 in tumors is seen, but it is a nonspecific finding; when present, it should be disregarded for diagnostic purposes.
甲状腺转录因子-1（TTF-1）的免疫组织化学鉴定被认为是细胞核染色模式的存在，并用于鉴定甲状腺或肺源性肿瘤。尽管已有关于TTF-1细胞质表达的报道，但这种模式的意义尚未得到详细研究。
确定TTF-1细胞质免疫染色的发生率，并分析这种免疫反应模式的诊断价值。
回顾了361例连续肿瘤病例的组织学切片，这些病例均进行了TTF-1染色，选择肿瘤细胞或非肿瘤细胞显示细胞质染色的病例。对后一组病例的肿瘤起源进行了临床相关性分析。
一家三级医疗机构的免疫组织化学实验室。
361个肿瘤来自29个器官部位，包括原发性和转移性肿瘤。23个（6.3%）肿瘤显示TTF-1的细胞质染色。其中13个肿瘤的原发部位得以明确：7个为肺癌（3个原发性肺腺癌、1个原发性大细胞癌、1个转移至肝脏的小细胞癌、1个转移至颈部淋巴结的腺癌和1个转移至大腿软组织的腺癌），3个结肠腺癌（2个转移至椎骨，1个转移至肺），1个转移至股骨的乳腺导管腺癌，1个转移至肝脏的喉鳞状细胞癌，以及1个累及眼眶骨的脑膜瘤。有3个肺癌伴有细胞核免疫染色。非肿瘤性肝组织是TTF-1抗体染色细胞质的最常见非肿瘤组织。
肿瘤中偶尔可见TTF-1的细胞质染色，但这是一个非特异性发现；出现这种情况时，在诊断时应予以忽略。
