原核生物对双精氨酸转运途径的利用：一项基因组调查。
Prokaryotic utilization of the twin-arginine translocation pathway: a genomic survey.
作者信息
Dilks Kieran, Rose R Wesley, Hartmann Enno, Pohlschröder Mechthild
机构信息
Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
出版信息
J Bacteriol. 2003 Feb;185(4):1478-83. doi: 10.1128/JB.185.4.1478-1483.2003.
Abstract
The twin-arginine translocation (Tat) pathway, which has been identified in plant chloroplasts and prokaryotes, allows for the secretion of folded proteins. However, the extent to which this pathway is used among the prokaryotes is not known. By using a genomic approach, a comprehensive list of putative Tat substrates for 84 diverse prokaryotes was established. Strikingly, the results indicate that the Tat pathway is utilized to highly varying extents. Furthermore, while many prokaryotes use this pathway predominantly for the secretion of redox proteins, analyses of the predicted substrates suggest that certain bacteria and archaea secrete mainly nonredox proteins via the Tat pathway. While no correlation was observed between the number of Tat machinery components encoded by an organism and the number of predicted Tat substrates, it was noted that the composition of this machinery was specific to phylogenetic taxa.
摘要
双精氨酸转运（Tat）途径已在植物叶绿体和原核生物中被发现，它允许折叠蛋白的分泌。然而，该途径在原核生物中的使用程度尚不清楚。通过使用基因组方法，建立了84种不同原核生物的假定Tat底物的综合列表。令人惊讶的是，结果表明Tat途径的使用程度差异很大。此外，虽然许多原核生物主要利用该途径分泌氧化还原蛋白，但对预测底物的分析表明，某些细菌和古细菌主要通过Tat途径分泌非氧化还原蛋白。虽然未观察到生物体编码的Tat机制组件数量与预测的Tat底物数量之间存在相关性，但注意到该机制的组成因系统发育类群而异。
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