Sawa Y, Horie Y, Yamaoka Y, Ebata N, Kim T, Yoshida S
Department of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, N13 W7, Kita-ku, Sapporo 060-8586, Japan.
J Dent Res. 2003 Feb;82(2):96-100. doi: 10.1177/154405910308200204.
Class II major histocompatilibity complex (MHC)-expressing cells are usually distributed in dental pulp, and it was postulated that the colony-stimulating factor (CSF) derived from dental pulp fibroblasts contributes to the migration of class II MHC-expressing cells into pulp tissue. This study aimed to investigate the CSF production of human dental pulp fibroblasts. In pulp tissue sections, granulocyte (G)-CSF was detected from normal teeth, while G-CSF, macrophage (M)-CSF, and granulocyte-macrophage (GM)-CSF were detected from teeth with dentinal caries. In cultured dental pulp fibroblasts, G-CSF was detected by immunostaining, immunoprecipitation, and ELISA, and mRNAs of G-CSF, M-CSF, and GM-CSF were detected by RT-PCR. The dental pulp fibroblasts cultured with TNF-alpha were found to increase the G-CSF expression and to produce M-CSF and GM-CSF. These findings suggest that dental pulp fibroblasts usually produce G-CSF. In the presence of TNF-alpha, dental pulp fibroblast express M-CSF and GM-CSF.
表达II类主要组织相容性复合体（MHC）的细胞通常分布于牙髓中，据推测，牙髓成纤维细胞产生的集落刺激因子（CSF）有助于表达II类MHC的细胞迁移至牙髓组织。本研究旨在调查人牙髓成纤维细胞的CSF产生情况。在牙髓组织切片中，从正常牙齿中检测到粒细胞（G）-CSF，而从患有牙本质龋的牙齿中检测到G-CSF、巨噬细胞（M）-CSF和粒细胞-巨噬细胞（GM）-CSF。在培养的牙髓成纤维细胞中，通过免疫染色、免疫沉淀和酶联免疫吸附测定法检测到G-CSF，并通过逆转录-聚合酶链反应（RT-PCR）检测到G-CSF、M-CSF和GM-CSF的信使核糖核酸（mRNA）。发现用肿瘤坏死因子-α（TNF-α）培养的牙髓成纤维细胞可增加G-CSF表达并产生M-CSF和GM-CSF。这些发现表明，牙髓成纤维细胞通常产生G-CSF。在TNF-α存在的情况下，牙髓成纤维细胞表达M-CSF和GM-CSF。
