Wang Y, Spencer P
Department of Oral Biology, University of Missouri-Kansas City School of Dentistry, 650 E. 25th Street, Kansas City, MO 64108, USA.
J Dent Res. 2003 Feb;82(2):141-5. doi: 10.1177/154405910308200213.
Although it is generally proposed that dentin bonding results from adhesive infiltration of superficially demineralized dentin, it is not clear how well the resin monomers seal the dentin collagen fibrils under wet bonding conditions. The aim of this study was to determine the quality and molecular structure of adhesive/dentin (a/d) interfaces formed with wet bonding as compared with adhesive-infiltrated demineralized dentin (AIDD) produced under controlled conditions (optimum hybrid). From each extracted, unerupted human 3rd molar, one fraction was demineralized, dehydrated, and infiltrated with Single Bond (SB) adhesive under optimum conditions; the remaining, adjacent fraction was treated with SB by wet bonding. AIDD and a/d interface sections were stained with Goldner's trichrome; corresponding sections were analyzed with micro-Raman spectroscopy. The histomorphologic and spectroscopic results suggest that, under wet bonding, the a/d interface is a porous collagen web infiltrated primarily by the hydrolytically unstable HEMA.
虽然一般认为牙本质粘结是由于树脂渗入表面脱矿的牙本质，但在湿粘结条件下树脂单体对牙本质胶原纤维的封闭效果尚不清楚。本研究的目的是确定与在可控条件下（最佳混合）制备的粘结剂渗入脱矿牙本质（AIDD）相比，湿粘结形成的粘结剂/牙本质（a/d）界面的质量和分子结构。从每颗拔除的未萌出人类第三磨牙中，一部分进行脱矿、脱水，并在最佳条件下用单键（SB）粘结剂渗入；其余相邻部分通过湿粘结用SB处理。AIDD和a/d界面切片用Goldner三色染色法染色；相应切片用显微拉曼光谱分析。组织形态学和光谱学结果表明，在湿粘结条件下，a/d界面是一个主要由水解不稳定的甲基丙烯酸羟乙酯渗入的多孔胶原网。
