Steinberg Francene, Stites Tracy E, Anderson Peter, Storms David, Chan Ivan, Eghbali Sheila, Rucker Robert
Department of Nutrition, University of California, Davis, California 95616, USA.
Exp Biol Med (Maywood). 2003 Feb;228(2):160-6. doi: 10.1177/153537020322800205.
Growth, reproductive performance, and indices of collagen maturation and expression were investigated in Balb/c mice fed chemically defined, amino acid-based diets with or without the addition 6 micro Mpyrroloquinoline quinone (PQQ)/kg diet. The diets were fed to virgin mice for 8 weeks before breeding. At weaning, the pups from successful pregnancies were fed the same diet as their respective dams. Reproductive performance was compromised in mice fed diets devoid of PQQ, and their offspring grew at slower rates than offspring from mice fed diets supplemented with PQQ. Successful mating (confirmed vaginal plugs) was not affected by the presence or absence of PQQ; however, pup viability (number of pups at parturition/number of pups at Day 4 of lactation) was decreased in PQQ-deprived mice. Conception (percentage of females giving live births) and fertility (percentage of births) were also decreased in PQQ-deprived mice. The slower rates of growth in offspring from PQQ-deprived mice were associated with decreased steady-state mRNA levels for Type I procollagen alpha(1)-chains in skin and lungs from neonatal mice. Values for lysyl oxidase accumulation as protein in PQQ-deficient mice also tended to be lower than corresponding values from PQQ-supplemented or -replete mice. Skin collagen solubility was increased in PQQ-deprived mice. These results indicate that PQQ supplementation can improve reproductive performance, growth, and may modulate indices of neonatal extracellular matrix production and maturation in mice fed chemically defined, but otherwise nutritionally complete diets.
在喂食化学定义的、基于氨基酸的日粮（添加或不添加6微克/千克饮食的吡咯喹啉醌（PQQ））的Balb/c小鼠中，研究了生长、繁殖性能以及胶原蛋白成熟和表达指标。在繁殖前，将这些日粮喂给未交配的小鼠8周。断奶时，成功怀孕的母鼠所生幼崽喂食与各自母鼠相同的日粮。喂食不含PQQ日粮的小鼠繁殖性能受到损害，其后代生长速度比喂食添加PQQ日粮的小鼠后代慢。PQQ的有无不影响成功交配（确认有阴道栓）；然而，缺乏PQQ的小鼠幼崽活力（分娩时幼崽数量/哺乳第4天幼崽数量）下降。缺乏PQQ的小鼠受孕率（产活仔雌性百分比）和生育率（出生百分比）也下降。缺乏PQQ的小鼠后代生长速度较慢与新生小鼠皮肤和肺中I型前胶原α(1)链的稳态mRNA水平降低有关。PQQ缺乏小鼠中作为蛋白质积累的赖氨酰氧化酶的值也往往低于PQQ补充或充足小鼠的相应值。缺乏PQQ的小鼠皮肤胶原蛋白溶解度增加。这些结果表明，补充PQQ可以改善繁殖性能、生长，并可能调节喂食化学定义但营养上完全的日粮的小鼠新生细胞外基质产生和成熟的指标。
