Zhang L, Yao H, Cao X, Yu Y, Chen H, Li M
First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(6):330-3.
To explore the anti-schistosomal hepatic fibrosis effect and the changes in transforming growth factor-beta 1 (TGF-beta 1) and its receptors (TGF-beta RII) in S. japonicum infected mice after intrasplenic transplantation of gamma-interferon (IFN-gamma) gene-modified hepatocytes.
At 16 wk after infection with cercariae of Schistosoma japonicum, the mice were intrasplenically transplantated with murine hepatocytes which had been transfected with IFN-gamma gene-combinant adenovirus vector. ELISA, immunohistochemical and dot blot techniques were used to observe the dynamic changes in IFN-gamma, TGF-beta 1, TGF-beta RII and type I, III collagen.
The intrasplenic transplantation of IFN-gamma gene modified hepatocytes effectively expressed IFN-gamma and obviously reduced the production and deposition of type I, III collagen as well as TGF-beta 1 and TGF-beta RII.
IFN-gamma gene transplantation has anti-hepatic fibrosis efficacy in Schistosoma japonicum-infected mice, being related to its role of decreasing the expression of TGF-beta 1 and TGF-beta RII.
探讨γ-干扰素（IFN-γ）基因修饰的肝细胞脾内移植对日本血吸虫感染小鼠抗血吸虫性肝纤维化的作用以及转化生长因子-β1（TGF-β1）及其受体（TGF-βRII）的变化。
日本血吸虫尾蚴感染小鼠16周后，将经IFN-γ基因重组腺病毒载体转染的小鼠肝细胞脾内移植。采用ELISA、免疫组化和斑点杂交技术观察IFN-γ、TGF-β1、TGF-βRII及I型、III型胶原的动态变化。
IFN-γ基因修饰的肝细胞脾内移植能有效表达IFN-γ，并明显减少I型、III型胶原以及TGF-β1和TGF-βRII的产生与沉积。
IFN-γ基因移植对日本血吸虫感染小鼠具有抗肝纤维化作用，这与其降低TGF-β1和TGF-βRII表达的作用有关。
