Bian Z, Wang G, Tian X, Fan J
Department of Gastroenterology, Kunming General Hospital, Kunming 650032.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(6):359-62.
To provide theoretical evidence for studying the molecular pathogenesis of human cerebral malaria.
The expressions of Plasmodium falciparum erythrocyte membrane protein 1(PfEMP1) on the surface of parasitized erythrocyte (PE) specimens from 19 cases of cerebral malaria patients in Yunnan Province were quantitatively analyzed by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) technique. 43 patients of falciparum malaria, 9 patients of vivax malaria and 6 healthy controls were also investigated.
The expressions of higher molecular mass (Mr) 260-320 kDa forms of PfEMP1 were found on PE from cerebral malaria patients. By contrast, the expression of PfEMP1 and P. vivax erythrocyte membrane protein (PvEMP1) on PE from falciparum malaria patients and vivax malaria patients had a PfEMP1 with Mr 240 kDa and a PvEMP1 with Mr 180 kDa band, respectively. Healthy controls expressed an EMP of Mr 140 kDa.
The binding of 260-320 kDa PfEMP1 proteins expressed on PE from cerebral malaria patients to diverse receptor molecules on the endothelial cell(EC) of the cerebral microvessels such as CD36, thrombospondin (TSP), intercellular adhesion molecule 1(ICAM-1), vascular cell adhesion molecule 1(VCAM-1), endothelial leukocyte adhesion molecule 1(ELAM-1) and chondroitin sulfate A (CSA) might be the molecular basis for the pathogenesis of cerebral malaria.
为研究人类脑型疟疾的分子发病机制提供理论依据。
采用制备性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）技术，对云南省19例脑型疟疾患者的寄生红细胞（PE）标本表面恶性疟原虫红细胞膜蛋白1（PfEMP1）的表达进行定量分析。同时对43例恶性疟患者、9例间日疟患者及6例健康对照者进行了研究。
在脑型疟疾患者的PE上发现了高分子量（Mr）260 - 320 kDa形式的PfEMP1表达。相比之下，恶性疟患者和间日疟患者的PE上PfEMP1和间日疟原虫红细胞膜蛋白（PvEMP1）的表达分别有一条Mr为240 kDa的PfEMP1带和一条Mr为180 kDa的PvEMP1带。健康对照者表达的EMP分子量为140 kDa。
脑型疟疾患者的PE上表达的260 - 320 kDa PfEMP1蛋白与脑微血管内皮细胞（EC）上的多种受体分子如CD36、血小板反应蛋白（TSP）、细胞间黏附分子1（ICAM-1）、血管细胞黏附分子1（VCAM-1）、内皮白细胞黏附分子1（ELAM-1）和硫酸软骨素A（CSA）的结合可能是脑型疟疾发病机制的分子基础。
