Shi H, Tian C, He D
Department of Parasitology, Guangxi Medical University, Nanning, 530021.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(4):200-2.
To observe whether there were any intra-species differences between Anopheles minimus from Hainan(H) and Yunnan(Y).
Anopheles minimus were collected from cattle shed on the spot. Each isofemale line was set up in the laboratory. Hybridization experiments were conducted by using forced mating between Anopheles minimus from Hainan and Yunnan, for observing the reproductive ability of F1 hybrids. Ovarian nurse cell polytene chromosomes of F1 hybrid females were examined, to observe any synapsis in different zones of chromosomes.
No embryo formation was found within the eggs produced by group Y female x H male, the hatching rate was zero. Low hatching rate was shown in other groups with (H female x Y male) F1, except for groups with (H x Y) F1 x Y. Ovarian nurse cell polytene chromosomes from (H x Y) F1 hybrid females showed constant asynapsis at the 29th, 36th and 37th zones in the chromosome 3R, and at the 4th and 6th zones in the chromosome X.
Reproductive isolation did appear in An. minimus from Hainan and Yunnan.
观察海南（H）和云南（Y）微小按蚊之间是否存在种内差异。
在现场牛棚采集微小按蚊。在实验室建立每个同雌系。通过海南和云南微小按蚊之间的强制交配进行杂交实验，以观察F1代杂种的生殖能力。检查F1代杂种雌蚊的卵巢滋养细胞多线染色体，观察染色体不同区域的联会情况。
Y雌×H雄组所产的卵内未发现胚胎形成，孵化率为零。其他（H雌×Y雄）F1组孵化率较低，（H×Y）F1×Y组除外。（H×Y）F1杂种雌蚊的卵巢滋养细胞多线染色体在3R染色体的第29、36和37区以及X染色体的第4和6区显示持续的异联会。
海南和云南的微小按蚊之间确实出现了生殖隔离。
