Shi Huan-huan, Huang Jie-gang, Tian Chun-lin, Yuan Zhi-gang
Department of Parasitology, Guangxi Medical University, Nanning 530021, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006 Jun;24(3):203, VI, inside back cover.
Anopheles minimus collected from Yuanjiang, Yunnan Province, were bred with standard methods in lab. The ovarian nurse cells of A.minimus were separated and stained, and the whole polytene chromosomes were photographed under light microscope and compared with A.minimus from Guangxi. 365 samples of ovarian nurse cells were observed. The chromosomes included one telocentric sex-chromosome X, two submetacentric autosomes II (autosome II right arm, 2R and autosome II left arm, 2L) and two metacentric autosomes III (autosome III right arm, 3R, and autosome III left arm, 3L). The X is the shortest chromosome and the 2R is the longest one. In comparison with the pattern of polytene chromosomes of A. minimus from Guangxi, difference at 12 positions has been found at the parts of arms in banding sequences.
从云南省元江采集的微小按蚊在实验室中采用标准方法进行饲养。分离并染色微小按蚊的卵巢滋养细胞，在光学显微镜下拍摄整个多线染色体，并与来自广西的微小按蚊进行比较。观察了365个卵巢滋养细胞样本。这些染色体包括一条端着丝粒性染色体X、两条亚中着丝粒常染色体II（常染色体II右臂，2R和常染色体II左臂，2L）和两条中着丝粒常染色体III（常染色体III右臂，3R和常染色体III左臂，3L）。X是最短的染色体，2R是最长的染色体。与来自广西的微小按蚊多线染色体模式相比，在带纹序列的臂部区域发现了12个位置的差异。
