Zhai Z, Mei J, You J, Yao M, Xiao S
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(4):231-4.
To observe the effect of artemether (Art) on nucleoside uptake and nucleic acid content in Schistosoma japonicum.
RNA and DNA contents of both male and female worms harbored in mice treated intragastrically (i.g.) with Art 300 mg/kg for 24 h or 48 h were determined, respectively. After in vivo drug treatment, the schistosomes recovered were in vitro maintained in drug-free medium containing [3H]adenosine, [5-(3)H] uridine or [methyl-3H]thymidine at a final concentration of 37 MBq/L or 74 MBq/L for 2 h or 4 h, the tritiated nucleoside uptake and incorporation into nucleic acid of schistosomes were measured.
The RNA and DNA contents of female worms recovered from the host 48 h after dosing were markedly decreased by 51.6% and 23.5%, respectively, while the RNA content of male worms showed 42.4% reduction. When the above-mentioned schistosomes were in vitro exposed to the tritiated nucleoside for 2 h or 4 h, apparent decrease in tritiated nucleoside uptake with reduction rates of 35.2%-50.1% was seen in female worms. The incorporation of [methyl-3H]thymidine into the female worm DNA 2 h after incubation was reduced by 71.4% while the incorporation of [3H]adenosine into the female worm RNA and DNA 4 h after incubation was reduced by 65.2% and 50.0%, respectively.
Art exhibited an apparent effect on the nucleic acid metabolism in schistosomes, especially in female worms.
观察蒿甲醚（Art）对日本血吸虫核苷摄取及核酸含量的影响。
分别测定经胃内给予300mg/kg蒿甲醚24小时或48小时的小鼠体内雌雄虫体的RNA和DNA含量。体内药物处理后，将回收的血吸虫在体外于终浓度为37MBq/L或74MBq/L的含[3H]腺苷、[5-(3)H]尿苷或[甲基-3H]胸苷的无药培养基中培养2小时或4小时，测定血吸虫对氚标记核苷的摄取及掺入核酸的情况。
给药48小时后从宿主体内回收的雌虫RNA和DNA含量分别显著降低了51.6%和23.5%，而雄虫RNA含量降低了42.4%。当上述血吸虫在体外暴露于氚标记核苷2小时或4小时时，雌虫对氚标记核苷的摄取明显减少，降低率为35.2%-50.1%。孵育2小时后，[甲基-3H]胸苷掺入雌虫DNA的量减少了71.4%，而孵育4小时后，[3H]腺苷掺入雌虫RNA和DNA的量分别减少了65.2%和50.0%。
蒿甲醚对血吸虫的核酸代谢有明显影响，尤其是对雌虫。
